FIG 2. Schematic diagram of parallel plate-type flow chamber used for applying flow shear stress to endothelial cells cultured on PS surface. PS-coated slide glass on which endothelial cells (BAECs) were confluently cultured was mounted together with a silicone rubber gasket to a lower PMMA plate. Flow shear stress was imposed on BAECs by circulating 1% PBS maintained at 37°C in reservoir by using pump.