Fig 6. Two new cortical lesions and a new deep WM lesion in a 44-year-old female patient with MS scanned at an interval of 3 years. Baseline image (A) and its CSEG (D). Coregistered second time-point image (B) and the registered CSEG (E). Subtraction image (time point 2 minus baseline (C) and its SSEG (F). Two cortical lesions (arrowheads) are misclassified as normal GM with the CSEG method, but the subtraction image clearly shows these lesions. In addition, a new lesion is seen in the left preventricular deep WM (arrow). The CSEG method (D and E) segments CSF (blue), GM (orange), lesion (yellow), and WM (green); and SSEG method (F) only segments new lesions (pink). Subtle artifacts are seen on the boundary of the brain surface due to slight misregistration. (In all of the images, the skull has been stripped by masking with ICC.)