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American Journal of Neuroradiology 28:864-869, May 2007
© 2007 American Society of Neuroradiology

INTERVENTIONAL

mRNA Expression in Rabbit Experimental Aneurysms: A Study Using Gene Chip Microarrays

W.I. Mangruma, F. Farassatib, R. Kadirvelc, C.P. Kolbertc, S. Raghavakaimalc, D. Dai, Y.H. Ding, D. Grillc, V.G. Khuranac and D.F. Kallmesc

a Iowa Methodist Medical Center, Des Moines, Iowa
b University of Minnesota, Minneapolis, Minn
c The Mayo Clinic, Rochester, Minn

Address correspondence to David F. Kallmes, MD, 200 First Street SW, Rochester, MN 55905; e-mail: kallmes.david{at}mayo.edu

BACKGROUND AND PURPOSE: The molecular characteristics of intracranial aneurysms are still poorly documented. A rabbit elastase aneurysm model has been helpful in the evaluation of devices and strategies involved in endovascular treatment of aneurysms. The goal of this project was to document the molecular changes, assessed by gene chip microarrays, associated with the creation of aneurysms in this model compared with the contralateral carotid artery.

MATERIALS AND METHODS: A microarray of rabbit genes of interest was constructed using rabbit nucleotide sequences from GenBank. Elastase-induced saccular aneurysms were created at the origin of the right common carotid artery in 4 rabbits. Twelve weeks after aneurysm creation, RNA was isolated from the aneurysm as well as the contralateral common carotid artery and used for microarray experiments. Reverse transcription-polymerase chain reaction (RT-PCR) was performed on 1 animal as a confirmatory test.

RESULTS: Ninety-six (46%) of 209 genes in the microarray were differentially expressed in the rabbit aneurysm compared with the contralateral common carotid artery. In general, differential gene expression followed specific molecular pathways. Similarities were found between rabbit aneurysms and human intracranial aneurysms, including increased metalloproteinase activity and decreased production of the extracellular matrix. RT-PCR results confirmed the differential expression found by the gene chip microarray.

CONCLUSIONS: The molecular characteristics of the rabbit elastase-induced saccular aneurysm are described. The rabbit aneurysm model shares some molecular features with human intracranial aneurysms. Future studies can use the rabbit model and the new rabbit gene chip microarray to study the molecular aspects of saccular aneurysms.




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R. Kadirvel, Y.-H. Ding, D. Dai, D.A. Lewis, S. Raghavakaimal, H.J. Cloft, and D.F. Kallmes
Gene Expression Profiling of Experimental Saccular Aneurysms Using Deoxyribonucleic Acid Microarrays
AJNR Am. J. Neuroradiol., September 1, 2008; 29(8): 1566 - 1569.
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Copyright © 2008 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X