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AJNR - American Journal of Neuroradiology

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American Journal of Neuroradiology 28:1586-1593, September 2007
DOI 10.3174/ajnr.A0593
© 2007 American Society of Neuroradiology

INTERVENTIONAL

Efficient Transmicrocatheter Delivery of Functional Fibroblasts with a Bioengineered Collagen Gel-Platinum Microcoil Complex: Toward the Development of Endovascular Cell Therapy for Cerebral Aneurysms

T. Abruzzo^a, T. Tun^b and A. Sambanis^b

^a Section of Interventional Neuroradiology, Department of Radiology and The Neuroscience Institute, University of Cincinnati Medical Center, Cincinnati, Ohio
^b School of Chemical Engineering and P.H. Parker Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Ga

Please address correspondence and requests for reprints to Todd Abruzzo, MD, c/o Editorial Office, University of Cincinnati College of Medicine, Department of Neurosurgery, 231 Albert Sabin Way, PO Box 670515, Cincinnati, OH 45267-0515; e-mail editor{at}mayfieldclinic.com

BACKGROUND AND PURPOSE: Endoaneurysmal implantation of fibroblasts may promote healing of aneurysms and reduce recanalization after therapeutic embolization. The purpose of our study was to develop a device for delivery of fibroblasts with use of current microcoil technology.

MATERIALS AND METHODS: Cell carrier devices and cell-free devices were fabricated by associating collagen gels (with or without fibroblasts) with platinum microcoils. During the propagation of control cell carrier devices for 1 week in culture, cell-mediated gel contraction (CMGC) occurred. Modified cell carrier devices created by glutaraldehyde cross-linking, ascorbate coculture, or extended CMGC were also characterized in vitro. Devices were deployed through microcatheters (533 µm lumen, 160 cm length). Gel retention, cell retention, cell death, and the ability to support local cell migration were analyzed in vitro.

RESULTS: Cell viability was reduced by glutaraldehyde cross-linking but not by microcatheter transit. During microcatheter transit, cell carrier devices liberated minimal particulate matter and cellular DNA. Liberated particulate matter was reduced by glutaraldehyde cross-linking (P < .05) and extended CMGC (P < .04). Only cell carrier devices treated with glutaraldehyde cross-linking did not exhibit cell migration after microcatheter transit. Passage of cell-free devices through microcatheters sheared off most of their collagen gel.

CONCLUSION: Collagen gel-platinum microcoil complexes can mediate efficient transmicrocatheter delivery of viable, migration-capable fibroblasts. CMGC is a necessary component of the process of gel stabilization that enables successful microcatheter transit. Although extended CMGC and glutaraldehyde cross-linking enhance gel stabilization, glutaraldehyde cross-linking decreases cell viability and migratory potential.

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