AJDRAJNR - American Journal of Neuroradiology

This Article
Right arrow Abstract Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mizuno, T.
Right arrow Articles by Ushio, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mizuno, T.
Right arrow Articles by Ushio, Y.

Single Blood Injection into the Ventral Cisterna Magna Through a Microcatheter for the Production of Delayed Cerebral Vasospasm: Experimental Study in Dogs

Takamasa Mizunoa, Jun-ichiro Hamadaa, Yutaka Kaia, Tatemi Todakaa, Motohiro Moriokaa and Yukitaka Ushioa

a From the Department of Neurosurgery, Kumamoto University School of Medicine, Japan



View larger version (59K):

[in a new window]
  		FIG 1. A, Lateral (left) conventional radiograph of the occipital cranium and cervical spine of a dog demonstrate the microcatheter and microguidewire inserted into the ventral CM. Right part of A is an enlargement of the rectangular area on the left.

B, Photograph of a gross specimen shows the ventral surface of the brain stem after injection of 0.5 ml/kg autologous fresh blood. The BA is completely embedded in clotted subarachnoid blood.



View larger version (172K):

[in a new window]
  		FIG 2. Representative serial angiograms of the canine BA.

A, Physiologic saline (0.5 ml/kg) was injected into the ventral CM. No constriction is noted during the 14-day observation period.

B, Autologous blood (0.25 ml/kg) was injected into the ventral CM. Severe constriction is present on day 3. Over time, the diameter of the BA gradually returns to normal.

C, Autologous blood (0.5 ml/kg) was injected into the ventral CM. There are sites with severe arterial constriction on days 3 and 7 after SAH induction; severe vasoconstriction persists on days 10 and 14.



View larger version (12K):

[in a new window]
  		FIG 3. Graphic presentation of the percent changes in the diameter of the BA on days 0 (before SAH), 3, 7, 10, and 14. Autologous fresh blood (0.5 or 0.25 ml/kg) or physiologic saline was injected into the ventral CM. Apparent arterial narrowing was observed in the 0.25 ml/kg SAH group on days 3, 7, 10, and 14 compared with the control group (* indicates P < .05). Arterial narrowing was more severe and persistent in the 0.5 ml/kg SAH group on days 7, 10, and 14 than in the the 0.25 ml/kg SAH group (** indicates P < .05). No arterial narrowing was apparent in the saline-injected control group.



View larger version (57K):

[in a new window]
  		FIG 4. Light-microscopic appearance of the BA removed at autopsy on the 7th post-SAH day (elastica van Gieson stain; original magnification, x100). Black bars in A and B indicate 100 µm.

A, Physiologic saline (0.5 ml/kg) was injected into the ventral CM. The diameter of the BA is normal.

B, Autologous blood (0.5 ml/kg) was injected into the ventral CM. There is severe narrowing of the vessel diameter, with folding and corrugation of the internal elastic lamina.





Copyright © 2008 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X