32P-Oligodeoxynucleotide-Coated Coils to Prevent Arterial Recanalization after Embolization
Luc Levesquea,
Francis Gauthiera,
Jean Raymondb and
Guy Leclerca
a Angiogene Inc., Montreal, Quebec, Canada
b Centre Hospitalier de L’Université de Montreal, 1560 Sherbrooke E, Montreal, Quebec, H2L 4M1
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FIG 1. Effect of 32P-oligodeoxynucleotide solution temperature on the activities of coils. Fragments of 1 cm of GDC-18 Soft (3 mm x 8 cm) coils were exposed to the 32P-oligodeoxynucleotide solution (0.8 µCi/µL) at various temperatures for 15 minutes. Coils were then washed and radioactivity levels were assessed. Values are means ± SEM of three experiments.
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FIG 2. Total radioactivity on coils as a function of length after immobilization with 32P-oligodeoxynucleotide solution. Entire GDCs were exposed to 32P-oligodeoxynucleotide solution (0.8 µCi/µL) heated at 65°C for 15 minutes. Coils were then washed and radioactivity levels were assessed. Values are means ± SEM of at least five experiments.
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FIG 3. In vitro retention profile of 32P-oligodeoxynucleotide. The remaining radioactivity (white circles) was compared with the natural decay for the 32P atom (black circles). Values are means ± SEM of duplicate experiments.
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FIG 4. In vivo retention profile of 32P-oligodeoxynucleotide.
A, Remaining radioactivity as a function of time of 32P-oligodeoxynucleotide-coated coils (white circles) compared with the natural decay of 32P (black circles).
B, Incorporation of 32P-oligodeoxynucleotide into thrombus (white circles) and arterial wall (black circles) after coil implantation. Values are means ± SEM of six experiments.
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FIG 5. Angiographic and pathologic findings after coil occlusion. Selected views from angiographic (A, B, and C), macroscopic (D, E, and F), and microscopic (G, H, and I) studies performed 12 weeks after standard platinum (A, D, and G) and radioactive coil (B, C, E, F, H, and I) implantation. Arteries treated with standard platinum coils were recanalized (D and G). Arteries treated with radioactive coils exhibited either subocclusions or total occlusions. Suboccluded arteries treated with radioactive coils (B, E, and H) had increased fibrous tissue content while permitting restricted blood flow (B, arrow). Occluded arteries treated with radioactive coils (C, F, and I) showed uniform fibrous filling of the lumen. Original magnification in D through F, x5; original magnification in G through I, x20.
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AJNR - American Journal of Neuroradiology