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Iron Particles Enhance Visualization of Experimental Gliomas with High-Resolution Sonography

Ingo Noltea,c, Giles H. Vinceb, Mathias Maurerc, Christian Herboldb, Roland Goldbrunnerb, Laszlo Solymosia, Guido Stollc and Martin Bendszusa

a Department of Neuroradiology, University of Würzburg, Germany
b Department of Neurosurgery, University of Würzburg, Germany
c Department of Neurology, University of Würzburg, Germany



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  		FIG 1. A–M, MR images (T2-weighted, 2500/96; T1-weighted, 600/14), sonograms (US), and photomicrographs of paraffin sections (hematoxylin-eosin stain; original magnification, X2) of C6 glioma in rat brain. Each row shows the different modalities in one animal. Images in the first row (A–D) were obtained without contrast media, the second row of images (E–H) was gadolinium-enhanced, and images in the third row (J–M) were SPIO-enhanced.

A–D, On the nonenhanced images, the C6 glioma is predominantly hyperintense on the T2-weighted image (A) and isointense on the T1-weighted image (B). On the sonogram (C), the tumor appears iso- to slightly hyperechoic to brain tissue. With hematoxylin-eosin staining (D), the corresponding area is clearly identifiable.

E–H, After injection of gadopentetate dimeglumine, the tumor is hyperintense on the T1-weighted image (F) and reveals similar characteristics on T2-weighted image (E), sonogram (G), and hematoxylin-eosin staining (H) to those of the tumor on nonenhanced images.

J–M, Twenty-four hours after injection of SPIO particles, the tumor is inhomogeneously hypo- to hyperintense on T2-weighted (J) and T1-weighted (K) images. On the sonogram (L), the tumor appears inhomogeneously hyperechoic to brain tissue with hyperechogic margins.



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  		FIG 2. A–D, Histologic and immunohistologic localization of iron, tumor cells, and macrophages. Photomicrographs (original magnification, X500 [A, C, and D] and X330 [B]) of paraffin sections stained with Perl’s stain (A and B) for detection of SPIO particles show iron-laden tumor cells in the center of the tumor in A and mostly mononuclear cells in the periphery of the tumor in B. These cells represent iron-labeled macrophages as shown by double labeling with the antibody ED-1 (C). The black-appearing (due to overlap of blue and brown staining product) macrophages in C show discernible blue staining for iron and brown ED-1 immunoreactivity in D when the same section is examined by using a different optic filter of the microscope (identical cells are marked by arrows).





Copyright © 2008 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X