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Modified Histologic Technique for Processing Metallic Coil-Bearing Tissue

Daying Daia, Yong Hong Dinga, Mark A. Danielsona, Ramanathan Kadirvela, Debra A. Lewisa, Harry J. Clofta and David F. Kallmesa

a From the Department of Radiology, Mayo Clinic, Rochester, MN



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  		FIG 1. Rabbit aneurysm, 2 weeks postembolization.

A, Photomicrograph shows unorganized thrombus within the aneurysm cavity and across the aneurysm neck. Excellent tissue preservation was achieved in this setting of friable thrombus. (H&E; original magnification, 20x).

B, Higher magnification of aneurysm neck shown in panel A. Photomicrograph shows no endothelial cell coverage at aneurysm neck (H&E; original magnification, 100x).



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  		FIG 2. Rabbit aneurysm, 10 weeks postembolization, illustrates loose connective tissue completely filling the aneurysm’s cavity; thin layer of tissue covered with single layer of endothelial cells traverses the entire neck. Excellent tissue preservation is achieved within the dome and at the neck (H&E; original magnification, 15x).



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  		FIG 3. Swine aneurysms, 1 week (A) and 12 weeks (B) postembolization.

A, Photomicrograph shows the unorganized thrombus without endothelial cell infiltration across the entire neck (H&E; original magnification, 40x).

B, Photomicrograph reveals substantial, chronic inflammatory changes in the dome, and a thick layer of hypercellular tissue across the aneurysm’s neck (H&E; original magnification, 20x). Excellent tissue preservation is achieved in both early and late time samples.



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  		FIG 4. Rabbit aneurysm, 16 weeks postembolization (A), and swine aneurysm, 12 weeks postembolization (B). Photomicrographs show Masson trichrome was successfully performed on both species. A, Masson trichrome; original magnification, 40x. B, Masson trichrome; original magnification, 60x.



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  		FIG 5. Rabbit aneurysm, 4 weeks postembolization (A), and swine aneurysm, 12 weeks postembolization (B). Photomicrographs show immunohistochemistry was successfully performed on coiled rabbit and swine tissues. Brown signal intensity localized within the spindle cells’ cytoplasm indicates positive staining. A, Antibody for Alpha smooth muscle actin antibody; original magnification, 400x. B, Antibody for desmin antibody; original magnification, 150x.





Copyright © 2009 by the American Society of Neuroradiology. Print ISSN: 0195-6108 Online ISSN: 1936-959X