Advanced

AJNR - American Journal of Neuroradiology

Published ahead of print on November 1, 2007
doi: 10.3174/ajnr.A0791

This Article Abstract Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vite, C.H. Articles by Poptani, H. Search for Related Content PubMed PubMed Citation Articles by Vite, C.H. Articles by Poptani, H.

Apparent Diffusion Coefficient Reveals Gray and White Matter Disease, and T2 Mapping Detects White Matter Disease in the Brain in Feline Alpha-Mannosidosis

C.H. Vite^a, S. Magnitsky^b, D. Aleman^a, P. O’Donnell^a, K. Cullen^a, W. Ding^a, S. Pickup^b, J.H. Wolfe^c and H. Poptani^b

^a W.F. Goodman Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pa
^b Department of Radiology, School of Medicine, University of Pennsylvania, Philadelphia, Pa
^c Stokes Institute, Children’s Hospital of Philadelphia, Philadelphia, Pa

View larger version (70K): [in this window] [in a new window]   Fig 1. A and B, ADCav maps of the brain at the level of the thalamus (A) and the cerebellum (B) are shown. The brain regions for which data were acquired include 4 gray matter regions (1 indicates cerebral cortex; 2, thalamus; 3, hippocampus; 4, cerebellar gray matter) and 4 white matter regions (5 indicates corona radiata; 6, centrum semiovale; 7, internal capsule; 8, cerebellar white matter).

View larger version (21K): [in this window] [in a new window]   Fig 2. T2 relaxation times of gray matter regions (cerebral cortex, thalamus, hippocampus, and cerebellar cortex and white matter regions (corona radiata, centrum semiovale, internal capsule, and cerebellar white matter [bottom]) were determined in unaffected and AMD cats. Significant increases in T2 were found in the white matter of AMD cats compared with unaffected cats (*P < .05). No significant differences in T2 were found between affected and normal cats when gray matter regions were compared.

View larger version (19K): [in this window] [in a new window]   Fig 3. ADCav of gray matter regions (cerebral cortex, thalamus, hippocampus, and cerebellar cortex and white matter regions (corona radiata, centrum semiovale, internal capsule, and cerebellar white matter [bottom]) were determined in unaffected and AMD cats. Significant decreases in ADVav were found in AMD cats in all gray and white matter regions examined (*P < .05).

View larger version (153K): [in this window] [in a new window]   Fig 4. A and B, H&E-stained sections of the cerebellar cortex (original magnification x400) from a normal cat (A) and an AMD cat (B), showing cytoplasmic vacuolation and distention of Purkinje cells (arrowhead), granular cells (small arrow), and glia (large arrow). C and D, Luxol fast blue staining of the cerebellar white matter (original magnification x50) from a normal cat (C) and an AMD cat (D). A decrease in myelination is seen as a decrease in stain intensity and as an increase in space between myelin sheaths. E and F, GFAP immunohistochemistry of the cerebellum (original magnification x200) from a normal (E) and an AMD cat (F), showing diffuse astrogliosis (brown staining) present throughout the gray and white matter of affected cats.