Published ahead of print on December 13, 2007
doi: 10.3174/ajnr.A0851
Apparent Diffusion Coefficient and Cerebral Blood Volume in Brain Gliomas: Relation to Tumor Cell Density and Tumor Microvessel Density Based on Stereotactic Biopsies
N. Sadeghia,
N. D'Haeneb,
C. Decaesteckerb,
M. Levivierc,
T. Metensa,
C. Marisb,
D. Wiklerc,d,
D. Baleriauxa,
I. Salmonb and
S. Goldmand
a Department of Radiology, Hôpital Erasme, Brussels, Belgium
b Department of Pathology, Hôpital Erasme, Brussels, Belgium
c Department of Neurosurgery, Hôpital Erasme, Brussels, Belgium
d PET/Biomedical Cyclotron Unit, Hôpital Erasme, Brussels, Belgium
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Fig 1. Histopathologic correlation of MR imaging data with stereotactic biopsy specimens in a 19-year-old patient with a right frontotemporal grade II astrocytoma. A–D, Top row shows axial 3D T1-weighted images (TR/TE, 20/4.6 ms) with contrast (A), coregistered ADC map (B), coregistered rCBV map (C), and corresponding histopathologic results for a "peritumoral tissue" sample (D). E–H, Bottom row shows axial 3D T1-weighted images (TR/TE, 20/4.6 ms) with contrast (E), coregistered ADC map (F), coregistered rCBV map (G), and corresponding histopathologic results for a "infiltrated tissue" sample (H). Regions of interest where ADC and rCBV have been measured are illustrated (arrows). The samples were immunohistochemically stained by using a monoclonal antibody against the CD34 antigen to assess microvessel density (arrows) and counterstained with hematoxylin to assess cell density (arrowheads) (original magnification, x 400).
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Fig 2. Histopathologic correlation of MR imaging data with stereotactic biopsy specimens in a 50-year-old patient with a right parietal grade IV astrocytoma. A–D, Top row shows axial 3D T1-weighted images (TR/TE, 20/4.6 ms) with contrast (A), coregistered ADC map (B), coregistered rCBV map (C), and corresponding histopathologic results for an "infiltrated tissue" sample (D). E–H, Bottom row shows axial 3D T1-weighted images (TR/TE, 20/4.6 ms) with contrast (E), coregistered ADC map (F), coregistered rCBV map (G), and corresponding histopathologic results for a "bulk tumor" sample (H). Regions of interest where ADC and rCBV have been measured are illustrated (arrows). The samples were immunohistochemically stained by using a monoclonal antibody against the CD34 antigen to assess microvessel density (arrows) and counterstained with hematoxylin to assess cell density (arrowheads) (original magnification, x 400).
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Fig 3. Scatterplots show a positive correlation between rCBV ratios and both cell density (n = 81, r = 0.37, P < .001) (A) and microvessel density (n = 81, r = 0.26, P < .05) (B) in the whole set of samples. There is no significant correlation between ADC ratios and either cell density (n = 81, r = 0.11, P = .34) (C) or microvessel density (n = 81, r = –0.20, P = .08) (D). Spearman rank correlation test was used, and P < .05 was considered to be statistically significant.
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Fig 4. Scatterplots show higher correlation between rCBV ratios and both cell density (n = 33, r = 0.57, P < .001) (A) and microvessel density (n = 33, r = 0.46, P < .01) (B) in bulk tumor than in the whole set of samples (Fig 3). In bulk tumor, there is no significant correlation between ADC ratios and cell density (n = 33, r = –0.20, P = .26) (C), whereas an inverse correlation between ADC and microvessel density is found (n = 33, r = –0.36, P < .05) (D). Spearman rank correlation test was used, and P < .05 was considered to be statistically significant.
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