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AJNR - American Journal of Neuroradiology

Published ahead of print on January 9, 2008
doi: 10.3174/ajnr.A0892

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Endovascular Treatment of Experimental Aneurysms with Use of Fibroblast Transfected with Replication-Deficient Adenovirus Containing Bone Morphogenetic Protein-13 Gene

D. Dai^a, Y.H. Ding^a, M.A. Danielson^a, R. Kadirvel^a, G.A. Helm^b, D.A. Lewis^a, H.J. Cloft^a and D.F. Kallmes^a

^a Neuroradiology Research Laboratory, Department of Radiology, Mayo Clinic, Rochester Minn
^b Department of Neurosurgery, University of Virginia, Charlottesville, Va

View larger version (34K): [in this window] [in a new window]   Fig 1. Gross photographs of neck tissue coverage. A, The 14-day coil loops in Ad-BMP-13–treated aneurysms are embedded within thin translucent membranous tissue, which completely covers the orifice of the neck. B, The Ad-BMP-13 1-month coil loops are embedded within a moderately thick membranous tissue, which completely covers the neck. C–D, The 14-day and 1-month PAs, respectively, show that the coil loops are barely visible at the neck orifice, and no membranous tissue is visible..

View larger version (32K): [in this window] [in a new window]   Fig 2. Photomicrographs of aneurysmal necks at 3 months. A, From the PA group, a thin neointima completely transverses the neck. The tissue appears to be concave to the aneurysm cavity (H&E, original magnification 40x). B, From the FBC group, there is a thin collagenous tissue that completely covers the neck (H&E, original magnification 60x). C, From the Ad-BMP-13 group, there is moderately thick collagenous tissue completely across the neck. (H&E, original magnification 60x). All of the tissue traversing the necks is lined with a monolayer of cells contiguous with the endothelium of the parent arteries.

View larger version (61K): [in this window] [in a new window]   Fig 3. Photomicrographs of collagen formation. A, From the PA group, there is scant collagen within the aneurysm dome at 1 month (Masson trichrome, original magnification 100x). B, from the FBC group, there is local collagen deposition at 3 months (Masson trichrome, original magnification 100x). C–D, Both from the Ad-BMP-13 group, C shows diffuse, collagenous fibers within the dome at 1 month (Masson trichrome, original magnification 100x), and D shows a denser, more mature collagen within the dome at 6 months (Masson trichrome, original magnification 150x)..

View larger version (23K): [in this window] [in a new window]   Fig 4. A bar chart of percent fibrosis or collagen with time and between groups. The asterisk (*) above a group indicates that it is significantly less (P < .05) than the Ad-BMP-13 group within that same time period.