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FIG 2. Light microscopy of transvascular delivery of iron oxide particles in the rat brain.
A, Histochemical staining for iron after delivery of Feridex (10 mg Fe/kg) with BBB disruption. Iron staining is increased in the disrupted right cerebral hemisphere compared with the contralateral nondisrupted left hemisphere, or saline controls. V = ventricle, LC = left corpus callosum and ventricle, RC = right corpus callosum (original magnification x20).
B, Capillary staining for iron in right cerebral hemisphere after Feridex delivery with BBB disruption is indicated by arrowheads. Arrows indicate staining of red blood cells (original magnification x50).
C, Histochemical staining for iron in rat brain 2 hours after delivery of MION (10 mg Fe/kg) with BBB disruption shows an increase in iron staining in the disrupted right cerebral hemisphere (RH) compared with the nondisrupted left hemisphere (LH). V indicates the ventricle (original magnification x20).
D, Cellular staining for iron (arrows) is widespread in the right cerebral hemisphere 24 hours after delivery of 10 mg/kg MION with BBB disruption. Arrowheads indicate capillary staining (original magnification x100).
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