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FIG 4. Cresyl violet and silver impregnation stain of the hilar region of the dentate gyrus.
A, Cresyl violet–stained sections in a control rat after imaging showing no loss of neurons. (M-molecular region, G-granule cell layer, H-hilus, CA3-pyramidal cells of the hippocampal CA3 layer).
B, Twenty-four hours after the induction of seizures, there is a pronounced loss of neurons in the hilus seen in cresyl violet–stained sections.
C, The silver degeneration stain in an adjacent section, from the same control animal in A, confirms the lack of neuronal loss within the hilus of the dentate gyrus. Neuronal degeneration can be visualized as darkly stained neurons; this control section lacks any darkly stained neurons.
D, The widespread loss of hilar neurons is confirmed after staining with silver degeneration methods. Arrowheads denote several neurons that are clearly in the process of degenerating. Note that the other regions, including the CA3 region, did not have significant neuronal degeneration. (Scale bar: 100 microns)
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