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FIG 1. Determination of the FS. Images are argon-laser fluorescence confocal micrographs of a CINC with a helical pitch of 250 µm obtained after monopulse exposure assay with an initial vascular smooth muscle cell (VSMC) concentration of 2.6 x 105 cells per milliliter (original magnification x40).
A, An oval ROI, represented by the white line, is manually drawn on the device surface to begin the analysis. Wide solid straight arrows indicate loops of the nitinol coil; curved arrows, collagen fibers; thin solid straight arrow, live cells; open arrow, dead cells.
B, A mask is applied to the image matrix outside the ROI, and the surface area within the ROI is calculated.
C, The live cell surface area within the ROI is calculated by subtracting red fluorescence and masking photon-deficient pixels within the ROI.
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