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FIG 6. Histologic images of BDA anterograde of labeled axons in the right RST.
A and B, Normal images at magnification X16 (A, bar = 1.0 mm) and at magnification X50 (B, bar = 0.1 mm) show numerous labeled axons in the right RST (arrow). There are a few labeled axons on the contralateral side (arrowhead in A), as approximately 10% of RST axons do not cross the midline. For orientation, a slit was made in the ventral left white matter before sectioning.
CE, Histologic images of the right RST in an animal with an unmodified fibroblast graft at magnification X50 (bar = 0.1 mm) obtained at 3.5 mm rostral (C), 1.5 mm rostral (D), and 1.0 mm rostral (E) to the transplant. Note that the number of labeled axons (arrow) decreases as images are obtained closer to the transplant.
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