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FIG 4. Apoptotic and lytic cell death in meningioma cells. After 1-hour treatment of meningioma cell lines with 900 mOsm of mannitol (A and B), normal saline (C), and 900 mOsm hypertonic saline (D), the cells are reacted with TUNEL reagents to detect programmed cell death. A rapidly proliferating meningioma cell line (A) demonstrates frequent apoptotic cells that are stained brown by the TUNEL reagents. There are very few ghosts of dissolved cells, which are marked by the arrows. More lytic (arrows) cell death than apoptotic cell death (brown cells) occurs in a slow-growing meningioma line (B). Normal saline and hypertonic saline controls reveal no significant cell death.
