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Fig 1. Dependency of collagen gel contraction on fibroblast attenuation and time. Collagen gel diameter measured at time point (Dm) plotted as percentage of the initial gel diameter (Di) over a 7-day period for cell carrier devices of varying initial fibroblast (cell) attenuation. Initial gel collagen concentration was 2 mg/mL for all cell carrier devices and control devices containing no cells. Each data point is an average of triplicate samples (except for cell attenuation of 2 x 107 cells/mL) (n = 2); error bars are SDs for each data series. Day 7: 107 cells/mL construct versus 106 cells/mL construct (P < .08); 107 cells/mL construct versus 105 cells/mL construct (P < .003); 107 cells/mL construct versus 2 x 107 cells/mL construct (P > .25) (printed with permission from the Mayfield Clinic).
