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Fig 1. The localization of the bioluminescence imaging signal intensity reflects the anatomic site of injection. A, Bioluminescence imaging scans 14 days after injection of 293T cells transduced with a lentiviral construct encoding enhanced green fluorescent protein (EGFP) and Fluc separated by the internal ribosome entry site (I) of the encephalomyocarditis virus (LV-EGFP-I-Fluc) showing a focus that is located above the injection site: caudal to the eyes and on the left side of the head for the left striatum (L Str), caudal to the eyes and on the right side for the right striatum (R Str), between the eyes for the olfactory bulb (OB), near the caudal edge of the skull for the substantia nigra (SN), and intermediate between R Str and SN for the globus pallidus (GP). These sites correspond to the expected locations on the basis of the injection coordinates. B, Ex vivo bioluminescence images of 1-mm-thick coronal sections show the localization of the signal intensity at the site of injection. The sections are numbered in the anteroposterior direction from the bulbus olfactorius1 to the cerebellum.8 C, Immunohistochemistry for EGFP confirms the site of injection. (Reprinted by permission from Macmillan Publishers: Deroose et al. Mol. Therapy 2006;14:423–31, copyright 2006).
