Published ahead of print on September 3, 2008
doi: 10.3174/ajnr.A1131
American Journal of Neuroradiology 29:1644-1648, October 2008
© 2008 American Society of Neuroradiology
Technical Note
BRAIN
Anomalous J-Modulation Effects on Amino Acids in Clinical 3T MR Spectroscopy
H.-S. Liua,b,
H.-W. Chunga,
C.-J. Juanb,
S.-Y. Tsaia,
C.-Y. Wanga,b,
C.-C. Chanc,
G.-S. Huangb,
M.-C. Choua,
C.-S. Leed,
C.-W. Koe,
N.-Y. Chof and
C.-Y. Chenb
a Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan
b Department of Radiology, Tri-Service General Hospital, Taipei, Taiwan
c Philips Medical Systems, Best, the Netherlands
d Department of Chemistry, Tamkang University, Taipei, Taiwan
e Department of Computer Science and Engineering, National Sun Yat-Sen University, Kaohsiung, Taiwan
f Institute of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan
Please address correspondence to Cheng-Yu Chen, MD, Department of Radiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China; e-mail: sandy0928{at}seed.net.tw
SUMMARY: The signal-intensity loss from anomalous J-modulation
effects due to chemical-shift displacement was investigated
on amino acid groups (alanine, valine, leucine, and isoleucine)
at 3T by using point-resolved
1H spectroscopy in patients with
brain abscess and phantom experiments. With a larger chemical
shift between methyl and methine resonances, alanine shows a
greater effect of signal-intensity cancellation compared with
other amino acids around 0.9 ppm, resulting in noninverted doublets
at a TE of 144 ms.
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Introduction
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High-field MR imaging systems such as 3T strength aid in the
detection of brain metabolites due to increased sensitivity.
However, the clinical 3T system also increases the spatial mismatch
of section-selective radio-frequency pulses due to a larger
chemical shift difference at higher fields and reduced radio-frequency
pulse bandwidths.
1–4 The issue of limited radio-frequency
bandwidths is especially prominent with the increasing popularity
of parallel imaging with array coils, whose receive-only function
demands radio-frequency pulse transmission from the generally
inefficient body coil. One frequently encountered challenge
of MR spectroscopy at high-field MR imaging systems is the anomalous
J-modulation effect on the weakly coupled AX
3 system, which
could hamper identification of the ideally inverted lactate
doublet at TE = 144 ms.
1–4
Although clinical investigations of the anomalous J-modulation effects have centered on lactate1–4 with theoretic analysis described clearly in a previous article,5 the possible occurrence arising from other AX3 spin systems such as alanine or other amino acids, valine, leucine, and isoleucine, overlaid around 0.9 ppm in brain abscess,6 to our knowledge, has never been reported. With similar coupling constants exerted on the methyl protons around 7 Hz,7 these metabolites containing methyl and methine groups also form a lactate-like coupled system with a TE-dependent phase evolution of the methyl resonance. In this study, we aimed to use both in vivo and in vitro experiments to show that the anomalous J-modulation effect analogous to lactate is also present for alanine, but to a much lesser extent for other amino acids, on a clinical 3T MR imaging scanner.
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Description of Technique and Results
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We performed experiments on a 3T scanner (Philips Medical Systems,
Best, the Netherlands) using the quadrature body (Q-body) coil
for transmission and an 8-channel phased-array modified sensitivity
encoding head coil for receiving, by using point-resolved
1H
spectroscopy sequence (PRESS). The Q-body coil had a maximum
transmission B1 field of 13.5 µT (
590 Hz). Single-voxel
MR spectral data were obtained with TR/TE = 2000/40, 144, and/or
288 ms with 128 acquisitions (total scanning time, 4 minutes
56 seconds). Two patients with abscesses underwent MR spectroscopic
examinations on this system. To investigate the anomalous J-modulation
effect on alanine and amino acid groups, we performed in vitro
experiments on four 1.5-L bottles of aqueous solution phantom
containing 10 mmol/L of alanine, valine, leucine, and isoleucine,
respectively. The phantom experiments were performed by using
parameters identical to those of the patients, except that the
voxel size was fixed at 25
x 25
x 25 mm
3.
Figs 1 and 2 show the data from a 19-year-old man and a 42-year-old woman with brain abscess, respectively. The doublet at 1.47 ppm due to the presence of alanine was clearly visible in the 40-ms and 288-ms TE MR spectra (Figs 1B and 2C). However, for the first patient (Fig 1C), there was a positive peak centered on the same resonance which, if from alanine, is expected to exhibit an opposite sign of resonance (ie, inverted doublet) at TE = 144 ms. The spectrum acquired from the second patient with TE = 144 ms (Fig 2B) also exhibited signal-intensity canceling, whereas the spectrum acquired with TE = 288 ms showed substantially larger and clearer alanine resonances than that with TE = 144 ms. The expected inverted doublet of alanine in the 144-ms TE spectra was hardly detectable. Similarly, both of these in vivo spectra also show no clearly identifiable phase inversion of lactate around 1.33 ppm in the TE = 144 ms spectra. In contrast, note that the amino acid groups near 0.9 ppm for the 2 subjects showed clearly inverted spectral lines in the TE = 144 ms spectra without noticeable reduction in signal intensity. The in vivo observations are consistent with results from the phantom experiments, in which an incomplete inverted phase at TE = 144 ms was seen for the methyl protons in alanine with reduced amplitude, as opposed to the strong signals in TE = 40 ms and TE = 288 ms spectra (Fig 3), whereas valine (Fig 4), leucine, and isoleucine (not shown) showed clear inverted peaks at TE = 144 ms for the methyl protons with an amplitude comparable with that in TE = 40 ms and TE = 288 ms spectra.
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Fig 1. Transverse T2 fluid-attenuated inversion recovery MR image (A) and single-voxel spectra obtained in a 19-year-old male patient with brain abscess by using PRESS localization at 3T with TE = 40 ms (B) and TE = 144 ms (C). Succ indicates succinate; Ac, acetate; Ala, alanine; Lac, lactate; Lip, lipids; AA, amino acids.
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Fig 2. Transverse postcontrast T1-weighted MR image (A) and single-voxel proton MR spectra obtained in a 42-year-old female patient with brain abscess by using PRESS localization at 3T with TE = 144 ms (B) and TE = 288 ms (C). Ala, indicates alanine; Lac, lactate; AA, amino acids.
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Fig 3. Spectra from a phantom that contained 10 mmol/L of alanine to illustrate the anomalous J-modulation effect of the PRESS sequence at TE = 144 ms obtained from a 3T scanner, with the identical setting in the receiver gain at TE = 144 (A) and TE = 288 ms (B). Note at 1.47 ppm the incompletely inverted phase and reduced amplitude of the methyl doublet in the TE = 144 ms spectrum, evidence of the presence of an anomalous J-modulation effect.
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Fig 4. Spectra from a phantom that contained only valine at TE = 40 ms (A) and TE = 144 ms (B). The complex doublets between 0.90 and 1.06 ppm show a clearly inverted phase at TE = 144 ms.
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Discussion
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In this study, we demonstrated that the anomalous J-modulation
effect analogous to lactate is also present for alanine on a
clinical 3T scanner. Our inference suggests that the degree
of reduction in sensitivity for alanine detection at TE = 144
ms is similar to that of lactate detection, due to a similar
chemical shift difference between the methyl and the methine
group protons (2.78 ppm or 36 Hz at 3T for lactate, and 2.31
ppm or 296 Hz at 3T for alanine, respectively).
7 On our system
in which the refocusing radio-frequency bandwidth is 590 Hz,
>50% of the methine protons within the section profile may
thus exceed the spectral width without experiencing radio-frequency
refocusing for each of the two 180° pulses.
3,5 Depending
on whether the methine protons within the excitation volume
experience the second and/or the third radio-frequency pulses
in the PRESS sequence, the excitation volume thus is divided
into 4 partial volumes, each with a different phase evolution
that leads to destructive interference and consequent signal-intensity
loss particularly prominent at TE = 144 ms (1–3, 5).
In contrast to the situation of alanine, the chemical shift difference between the methyl and methine resonances is approximately 1.23 ppm (157 Hz at 3T) for valine and 0.70 ppm (89 Hz at 3T) for leucine, respectively,7 more than a factor of 2 smaller than that found for lactate. Consequently, the signal-intensity cancellation effect is anticipated to be substantially weaker than that for lactate and alanine. In other words, spectra acquired with TE = 144 ms may not show clearly noticeable anomalous J-modulation for the amino acids near the 0.90-ppm region in the spectra, as shown in both our in vivo and in vitro data.
Alanine is potentially a pathologic marker of abscess resulting from bacterial infections of all kinds.6 It has also been observed following ischemia8 or in meningioma.9 Although alanine is not regarded as the most important biomarker for the differential diagnosis between tumor and abscess,8 a number of animal studies have addressed its potential role in monitoring metabolic alterations following brain injuries.10,11 Furthermore, the concentration of alanine in the brain, if detectable by in vivo MR spectroscopy, is usually small compared with that of lactate in the presence of prominent anaerobic glycolysis. This means that with destructive signal-intensity loss due to anomalous J-modulation effects, the identification of alanine at TE = 144 ms by using PRESS is even more prone to error than the situation expected for lactate. Special care in spectral interpretation related to alanine is therefore recommended at high-field systems such as 3T.
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Footnotes
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Supported in part by National Science Council under grants NSC-95-2314-B-002-221-MY3
and NSC-95-2314-B-016-036-MY2.
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References
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Received September 30, 2007;
accepted after revision December 11, 2007.
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Introduction