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Research ArticlePharmacology Vignette

Natalizumab (Tysabri)

D.T. Selewski, G.V. Shah, B.M. Segal, P.A. Rajdev and S.K. Mukherji
American Journal of Neuroradiology October 2010, 31 (9) 1588-1590; DOI: https://doi.org/10.3174/ajnr.A2226
D.T. Selewski
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G.V. Shah
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B.M. Segal
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P.A. Rajdev
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S.K. Mukherji
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Abstract

SUMMARY: Natalizumab is a humanized IgG4κ monoclonal antibody that is a selective adhesion molecule inhibitor, which prevents adhesion of leukocytes to endothelial cells. It is the first monoclonal antibody approved by the FDA for the treatment of relapsing-remitting MS. This article will review the mechanism of action and clinical role of this agent.

Abbreviations

FDA
US Food and Drug Administration
FLAIR
fluid-attenuated inversion recovery
FSE
fast spin-echo
IgG
immunoglobulin G
MAdCAM-1
mucosal adressin cell adhesion molecule
MS
multiple sclerosis
PML
progressive multifocal leukoencephalopathy
TOUCH
Tysabri Outreach Unified Commitment to Health
VCAM -1
vascular cell adhesion molecule-1

Natalizumab (Tysabri; Biogen Idec, Cambridge, Massachusetts and Elan Pharmaceuticals, Dublin, Ireland) was the first FDA-approved monoclonal antibody for the treatment of MS. Natalizumab received FDA approval in 2004 for treatment of relapsing-remitting MS based on the AFFIRM and SENTINEL phase 3 clinical trials.1,2 More recently, the indications for natalizumab were expanded to include Crohn disease.3–5 In 2005, natalizumab was removed from the market following cases of PML but was reintroduced by the FDA with a mandatory surveillance program in 2006 called TOUCH (Biogen).6

Proposed Mechanism of Action

Natalizumab is a humanized monoclonal IgG4κ antibody that selectively binds to the α4-integrin component of adhesion molecules found on lymphocytes, monocytes, and eosinophils.7 α4-integrin is a subunit of the leukocyte adhesion molecules α4β1 and α4β7. In 1991, Yednock et al8 showed that targeting α4-integrin could prevent the development of demyelinating lesions in a mouse model of MS, elucidating the therapeutic potential for this medication.9 Natalizumab inhibits the interaction of α4β1 with VCAM-1 and of α4β7 with MAdCAM-1.10 VCAM-1 and MAdCAM-1 are found on endothelial cells and interact with α4β1 and α4β7 on leukocytes for firm adherence of leukocytes to endothelial cells, a requisite step for their extravasation into inflamed tissue (Fig 1).11 Natalizumab prevents migration of autoreactive leukocytes out of blood vessels into target organs by blocking the adhesion to endothelial cells of the α4-integrin component of adhesion molecules on leukocytes, inhibiting inflammation (Fig 2). Because VCAM-1 is expressed on inflamed cerebrovascular endothelial cells, α4β1 is believed to be the critical target of natalizumab in preventing leukocyte migration into the central nervous system in MS. In contrast, both VCAM-1 and MAdCAM-1 are upregulated on intestinal endothelium in Crohn disease. The efficacy of natalizumab in Crohn disease very likely is due to blockade of leukocyte adhesion factors α4β1 and α4β7 in tandem.4,7,10

Fig 1.
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Fig 1.

The normal process of leukocyte migration out of blood vessels into tissue involves interactions between leukocytes and endothelial cells including rolling (A), adhesion (B), and extravasation (C). The adhesion molecules α4β1 and α4β7 found on leukocytes are integral in the adhesion process to endothelial cells.

Fig 2.
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Fig 2.

A, Natalizumab blocks the adhesion of leukocytes to endothelial cells by blocking the interaction of the α4-integrin subunit of α4β1 with VCAM-1 and of α4β7 with mucosal MAdCAM-1. B, This prevents autoreactive leukocytes from exiting blood vessels and entering target organs to cause inflammation.

Clinical Indications

Natalizumab is approved for treatment of relapsing-remitting MS and Crohn disease. It is generally reserved for patients who fail first-line therapies (Figs 3 and 4).12 It must be given in conjunction with the TOUCH program, which is a national risk-minimization program designed to “minimize the risk of PML, minimize death and disability due to PML, and promote informed risk-benefit decisions regarding Natalizumab use.”6 This drug is being investigated for use in ulcerative colitis.

Fig 3.
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Fig 3.

A 27-year-old man presented with numbness and weakness of both upper extremities and the left lower extremity, with multiple enhancing MR imaging lesions. He was prescribed high-dose β interferon (Rebif) soon after his initial clinical exacerbation but was switched to glatiramer acetate 1 year later due to breakthrough radiologic disease activity. The patient developed new right-sided paresthesias 3 years after his initial presentation. The MR imaging in this figure was performed when the new symptoms developed. A, FLAIR-weighted sagittal FSE image of the brain shows patchy high-signal-intensity areas involving the corpus callosum, brain stem structures, and cerebellum. B, FLAIR-weighted axial FSE image shows multiple patchy areas of high FLAIR signal intensity involving the corpus callosum and bilateral periventricular white matter with the presence of edema around a large right periatrial lesion. C, Postcontrast T1-weighted axial FSE image shows that a majority of the larger lesions exhibit intense patchy enhancement, suggestive of active demyelination.

Fig 4.
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Fig 4.

Within 1 month of the MR imaging shown in Fig 3, the patient was started on a course of natalizumab (Tysabri), 300 mg administered intravenously every 4 weeks. Repeat MR imaging after 6 months was performed. A, FLAIR-weighted sagittal FSE image of the brain shows improvement in the patchy high-signal-intensity area of the corpus callosum with resolution of lesions involving the brain stem structures and cerebellum. B, FLAIR-weighted axial FSE image shows marked improvement in the areas of demyelination involving the corpus callosum and bilateral periventricular white matter. C, Postcontrast T1-weighted axial FSE image shows only 1 small area of enhancement in the right periventricular white matter, with lack of enhancement of the rest of the enhancing lesions.

Administration

Natalizumab is administered at specialized infusion centers enrolled in the TOUCH program for 1 hour with 1-hour monitoring. The TOUCH program also mandates regular appointments and follow-ups to monitor for signs of PML. The half-life is 11 days after 6 months of therapy.13

Side Effects

Reported serious side effects include the following:

  • Neutralizing antibody development: occurs in ≤10% of patients.1,4,5,14

  • Transfusion reaction/hypersensitivity: transfusion reactions ranging in severity from minor to anaphylaxis have been reported.1,2,12 These reactions are more common in patients with neutralizing antibodies.5

  • PML: a serious central nervous system infection due to JC virus, cases have been reported in patients with MS and Crohn disease.15 Natalizumab is recommended for use as a monotherapy in MS12 and Crohn disease to minimize risk.

  • Hepatotoxicity: clinically significant liver injury has been reported in 6 individuals without long-term liver failure.16

  • Opportunistic infection: viral encephalitis, cytomegalovirus, aspergillosis, cryptosporidium diarrhea, pneumocystis pneumonia, mycobacterium avium intracellulare, and Burkholderia cepacia pneumonia have been reported.17

  • Malignant melanoma: reports of malignant melanoma following therapy.18,19

Economic Issues

Natalizumab is given at 300 mg intravenously every 4 weeks for Crohn disease and MS. The cost is roughly $2800 per vial (300 mg) with an annual cost of $34,000. This is covered by most insurance companies.

References

  1. 1.↵
    1. Rudick RA,
    2. Stuart WH,
    3. Calabresi PA,
    4. et al
    . Natalizumab plus interferon beta-1a for relapsing multiple sclerosis. N Engl J Med 2006; 354: 911– 23
    CrossRefPubMed
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    1. Polman CH,
    2. O'Connor PW,
    3. Havrdova,
    4. et al
    . A randomized, placebo-controlled trial of natalizumab for relapsing multiple sclerosis. N Engl J Med 2006; 354: 899– 910
    CrossRefPubMed
  3. 3.↵
    1. Gordon FH,
    2. Lai CW,
    3. Hamilton MI,
    4. et al
    . A randomized placebo-controlled trial of a humanized monoclonal antibody to alpha4 integrin in active Crohn's disease. Gastroenterology 2001; 121: 268– 74
    CrossRefPubMed
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    1. Ghosh S,
    2. Goldin E,
    3. Gordon FH,
    4. et al
    . Natalizumab for active Crohn's disease. N Engl J Med 2003; 348: 24– 32
    CrossRefPubMed
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    1. Sandborn WJ,
    2. Colombel JF,
    3. Enns R,
    4. et al
    . Natalizumab induction and maintenance therapy for Crohn's disease. N Engl J Med 2005; 353: 1912– 25
    CrossRefPubMed
  6. 6.↵
    Department of Health and Human Services. Tysabri risk minimization action plan: summary of TOUCH. http://www.fda.gov/downloads/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/UCM107197.pdf. Accessed June 28, 2010
  7. 7.↵
    1. Lobb RR,
    2. Hemler ME
    . The pathophysiologic role of alpha 4 integrins in vivo. J Clin Invest 1994; 94: 1722– 28
    PubMed
  8. 8.↵
    1. Yednock TA,
    2. Cannon C,
    3. Fritz LC,
    4. et al
    . Prevention of experimental autoimmune encephalomyelitis by antibodies against alpha 4 beta 1 integrin. Nature 1992; 356: 63– 66
    CrossRefPubMed
  9. 9.↵
    1. Brocke S,
    2. Piercy C,
    3. Steinman L,
    4. et al
    . Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment. Proc Natl Acad Sci U S A 1999; 96: 6896– 901
    Abstract/FREE Full Text
  10. 10.↵
    1. Rice GP,
    2. Hartung HP,
    3. Calabresi PA
    . Anti-alpha4 integrin therapy for multiple sclerosis: mechanisms and rationale. Neurology 2005; 64: 1336– 42
    Abstract/FREE Full Text
  11. 11.↵
    1. Ley K,
    2. Laudanna C,
    3. Cybulsky MI,
    4. et al
    . Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat Rev Immunol 2007; 7: 678– 89
    CrossRefPubMed
  12. 12.↵
    1. Goodin DS,
    2. Cohen BA,
    3. O'Connor P,
    4. et al
    . Assessment: the use of natalizumab (Tysabri) for the treatment of multiple sclerosis (an evidence-based review): report of the Therapeutics and Technology Assessment Subcommittee of the American Academy of Neurology. Neurology 2008; 71: 766– 73
    Abstract/FREE Full Text
  13. 13.↵
    1. Rudick RA,
    2. Sandrock A
    . Natalizumab: alpha 4-integrin antagonist selective adhesion molecule inhibitors for MS. Expert Rev Neurother 2004; 4: 571– 80
    CrossRefPubMed
  14. 14.↵
    1. Miller DH,
    2. Khan OA,
    3. Sheremata WA,
    4. et al
    . A controlled trial of natalizumab for relapsing multiple sclerosis. N Engl J Med 2003; 348: 15– 23
    CrossRefPubMed
  15. 15.↵
    1. Clifford DB,
    2. DeLuca A,
    3. Simpson DM,
    4. et al
    . Natalizumab-associated progressive multifocal leukoencephalopathy in patients with multiple sclerosis: lessons from 28 cases. Lancet Neurol 2010; 9: 438– 46
    CrossRefPubMed
  16. 16.↵
    1. Bezabeh S,
    2. Flowers CM,
    3. Kortepeter C,
    4. et al
    . Review article: clinically significant liver injury in patients treated with natalizumab (TYSABRI). Aliment Pharmacol Ther 2010; 31: 1028– 35. Epub 2010 Feb 16
    PubMed
  17. 17.↵
    Center for Drug Evaluation and Research (CDER). Tysabri (Natalizumab) biologic license application 125104/15. In: Proceedings of the Peripheral and Central Nervous System Drugs Advisory Committee, Gaithersburg, Maryland. 3 7–8, 2006; available at http://www.fda.gov/ohrms/dockets/ac/06/slides/2006-4208S1-Slide-Index.htm. Accessed June 28, 2010
  18. 18.↵
    1. Ismail A,
    2. Kemp J,
    3. Sharrack B
    . Melanoma complicating treatment with natalizumab (Tysabri) for multiple sclerosis. J Neurol 2009; 256: 1771– 72. Epub 2009 Jul 16
    CrossRefPubMed
  19. 19.↵
    1. Mullen JT,
    2. Vartanian TK,
    3. Atkins MB
    . Melanoma complicating treatment with natalizumab for multiple sclerosis. N Engl J Med 2008; 358: 647– 48
    CrossRefPubMed
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Natalizumab (Tysabri)
D.T. Selewski, G.V. Shah, B.M. Segal, P.A. Rajdev, S.K. Mukherji
American Journal of Neuroradiology Oct 2010, 31 (9) 1588-1590; DOI: 10.3174/ajnr.A2226

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Natalizumab (Tysabri)
D.T. Selewski, G.V. Shah, B.M. Segal, P.A. Rajdev, S.K. Mukherji
American Journal of Neuroradiology Oct 2010, 31 (9) 1588-1590; DOI: 10.3174/ajnr.A2226
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