Data supplements
Supplemental Online Figures and Videos
Files in this Data Supplement:
- Online Figures 1-11 (PDF) -
Online Figure 1: Static image of MPA, prior to mixing with LA or plasma, at ×200 magnification. This demonstrates the crystalline nature of MPA and the tendency to form crystal aggregates. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 2: Static image of MPA, prior to mixing with LA or plasma, at ×400 magnification. This demonstrates the typical range in size of MPA crystals. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 3: Static image of MPA, after mixing with LA but without plasma, at ×400 magnification. This demonstrates that MPA crystals do not change in size, morphology or tendency to aggregate after mixing with LA. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 4: Static image of MPA, after mixing with LA and plasma, at ×200 magnification. This demonstrates that MPA crystals do not change in size or morphology after mixing with plasma however there is a reduction in the tendency of MPA crystals to aggregate. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 5: Static image of MPA, after mixing with LA and plasma, at ×400 magnification. At this magnification the individual MPA crystals are better seen and are demonstrated to not change in size or morphology after mixing with plasma. In addition there is a reduction in the tendency of MPA crystals to aggregate when mixed with plasma. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 6: Freeze frame from Video 1 of MPA flowing through a 200 micron depth channel after mixing with LA. This demonstrates the large size of MPA aggregates prior to mixing with plasma. In addition it confirms that as crystal aggregates maintain their integrity in a dynamic environment they have embolization potential. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification. There is a separate scale bar on the left side of the image that is somewhat obscured.
Online Figure 7: Static image of TA, prior to mixing with LA or plasma, at ×100 magnification. At this low magnification the large range in the size of TA crystals can be seen. In addition a tendency to aggregate can also be identified. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 8: Static image of TA, prior to mixing with LA or plasma, at ×400 magnification. At this high magnification the large size and rectangular brick like shape of TA crystals can be appreciated. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 9: Static image of TA, after mixing with LA and plasma, at ×100 magnification. This demonstrates that TA crystals do not change in size or morphology after mixing with plasma however there is a reduction in the tendency of crystals to aggregate. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Online Figure 10: Freeze frame from video 3 of TA flowing through a 200 micron depth channel after mixing with LA. This demonstrates the high number of large crystals in TA and also the tendency of small crystals to aggregate. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification. There is a separate scale bar on the left side of the image.
Online Figure 11: Static image of DSP, after mixing with LA, at ×100 magnification. The straight line on the left side of the image is the edge of the DSP droplet. As this is in focus it confirms we are focused at the correct depth to visualize any particulates that may be present. To the right of this line is the DSP preparation. There are no crystals or particulates. The white scale bar in the bottom right of the image represents 100 microns in length. The adjacent red circle represents the average size of a red blood cell at this magnification.
Right click videos and save as file. VLC media player is a free open-source media player that can display this file format. - Video 1 (MP4, 3.13 MB)
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Grayscale video of MPA flowing through a 200 micron depth channel after mixing with LA. This demonstrates the large size
of MPA aggregates prior to mixing with plasma. There is a scale bar on the left side of the image representing a length of
100 microns.
- Video 2 (MP4, 3.07 MB)
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Grayscale video of MPA flowing through a 200 micron depth channel after mixing with LA and plasma. This demonstrates the
reduction in size of MPA aggregates after mixing with plasma. There is a scale bar on the left side of the image representing
a length of 100 microns.
- Video 3 (MP4, 4.29 MB)
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Grayscale video of TA flowing through a 200 micron depth channel after mixing with LA. This demonstrates the high number
of large crystals in TA and also the tendency of small crystals to aggregate. There is a scale bar on the left side of the
image representing a length of 100 microns.
- Video 4 (MP4, 4.59 MB)
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Grayscale video of TA flowing through a 200 micron depth channel after mixing with LA and plasma. This demonstrates the
near complete absence of crystal aggregation after mixing with plasma. There is a scale bar on the left side of the image
representing a length of 100 microns.
- Video 5 (MP4, 532 KB)
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Grayscale video of DSP flowing through a 200 micron depth channel after mixing with LA. The straight line at the top of
the image is the edge of the channel. Apart from tiny particles of dust that likely reflect the environment that the samples
were prepared in, there are no crystals or significant particulates identified.
- Video 6 (MP4, 544KB)
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Grayscale video of DSP flowing through a 200 micron depth channel after mixing with LA and plasma. The straight line at
the top of the image is the edge of the channel. Apart from tiny residual red and white blood cells within the centrifuged
plasma there are no crystals or significant particulates are identified.
- Online Figures 1-11 (PDF) -