Intraobserver and Interobserver Variability for Measuring the Wall Area of the Basilar Artery at the Level of the Trigeminal Ganglion on High-Resolution MR Images

SUMMARY: We evaluated the intraobserver and interobserver variability for WA measurement of an atherosclerotic BA by using HRMRI. Ten consecutive patients underwent HRMRI, and the WA of the BA at the level of the trigeminal ganglion was measured by 2 independent readers and repeated by 1 reader 1 month later. There was strong agreement for intraobserver and interobserver measurements in the ICC and by the Bland-Altman method. Measurement of the WA in an atherosclerotic BA by HRMRI was reproducible.

P laque burden, represented by WA, has substantial usefulness in the assessment of atherosclerosis. 1 Imaging modalities used to measure plaque burden of the aorta and coronary, carotid, and peripheral arteries include intravascular sonography, 2 multidetector CT, 3 B-mode sonography determination of arterial intima media thickening, 4 and MR imaging. 5 HRMRI has advantages over other modalities in depicting the wall of the BA because it is noninvasive and radiation-free and lacks bone artifacts in the posterior fossa. 6,7 It also provides excellent boundary definition for BA-CSF and bloodintima borders, which allows a more precise measurement of the WA of the BA. However, to our knowledge, the reproducibility of such measurements by HRMRI has not been addressed. Our goal was to evaluate the intraobserver and interobserver variability of WA measurements of an atherosclerotic BA by using HRMRI.

Subjects
This prospective cohort study was approved by our institutional ethics committee, and written informed consent was obtained before HRMRI examination. Eligible patients had the following characteristics: 1) ischemic stroke or TIA of the posterior circulation within 90 days, 2) BA stenosis Ն70% on conventional angiography, and 3) Ն2 atherosclerotic risk factors (hypertension, hyperlipidemia, diabetes mellitus, cigarette smoking, and obesity). Hyperlipidemia was defined as a total cholesterol level Ն240 mg/dL, low-attenuation lipoprotein cholesterol level of Ն160 mg/dL, or current use of medication for lowering blood cholesterol levels.
We excluded patients with following conditions: 1) arteritis, 2) claustrophobia or contraindications to MR imaging such as the presence of ferromagnetic implants, 3) medically unable to remain in the MR imaging scanner for Ͼ40 minutes, and 4) Ͻ2 atherosclerotic risk factors.

Measurement of the WA of the BA
An image-quality rating (1 ϭ poor, 2 ϭ adequate, 3 ϭ good) was performed by a radiologist (X.L.) for each image. Patients with poor images due to subject motion or low signal intensity-to-noise ratio were excluded. The BA cross-sectional area at the level of trigeminal ganglion was measured by readers blinded to the clinical presenta-tion, by using research software (FuncTool 2 software, GE Healthcare) on the ADW4.3 workstation (Sun Microsystems, Santa Clara, California). The BA-CSF border was used to manually trace the vessel area and the blood-intima boundary for the lumen area. The WA was calculated as the difference between vessel area and lumen area.
To assess intraobserver variability, reader 1 (X.L.) measured the WA at the level of trigeminal ganglion, with the second set of measurements performed 1 month later. To examine interobserver variability, reader 2 (N.M.) determined the WA at the same level and compared the measurements with the first set obtained by reader 1.

Data Analysis
The ICC and the lower and upper limits the of the 95% CI were determined for intraobserver and interobserver variability. The Bland-Altman method for comparing paired measurements on T1WI, PDWI, T2WI, and postcontrast T1WI was used to determine intraobserver and interobserver agreement.

Results
Between June 2008 and July 2009, 10 consecutive male patients with symptomatic BA atherosclerosis underwent 3T HRMRI. Their mean age was 55 years (range, 45-72 years). All patients had 2 (n ϭ 5) or more (n ϭ 5) atherosclerotic risk factors. The qualifying event was posterior circulation ischemic stroke in 1 and TIA in 9 within 90 days. Image-quality ratings of all images were adequate-to-good. Thus, WA measurements of the BA at the level of trigeminal ganglion were available for analysis in all patients (Fig 1).
The intraobserver variability was small for measuring WA on T1WI, PDWI, T2WI, and postcontrast T1WI, with the ICC ranging from 0.973 to 0.980 (Table). The interobserver variability was also small on these sequences, with the ICC ranging from 0.957 to 0.989 (Table).

Discussion
This study demonstrates that HRMRI can be used to measure the plaque burden of BA atherosclerosis at the level of trigeminal ganglion with high reproducibility. For this pilot study, we chose the level at the trigeminal ganglion for its easy identification on MR images so that the measurements could be obtained as consistently as possible.
Postcontrast T1WI yields better reproducibility for WA measurements than T1WI, PDWI, and T2WI because the outer wall boundary is more clearly delineated. This is similar to previous findings in a carotid atherosclerosis study, 8 because the arterial wall, including the adventitia layer, is better penetrated by gadolinium-based contrast agents marking the outer wall boundary. To help delineate the vessel wall on PDWI and T2WI sequences, flow compensation and the inferior spatial saturation band were used in our study to suppress the inflow blood signal intensity and to minimize pulsation artifacts. Although the intraobserver and interobserver variability was greater on PDWI and T2WI sequences, it was still acceptable. However, these sequences have the advantage of requiring no contrast and less scanning time. Although obvious calcium in the vessel wall was not encountered in our patients, manually tracing the vessel area is difficult when calcium is present, especially on TIWI, because both CSF and calcium are hypointense. PDWI and T2WI may be more useful for distinguishing the vessel lumen in the presence of calcified plaque because CSF is hyperintense and calcium is hypointense.
HRMRI can potentially be used to monitor the progression or regression of BA atherosclerotic plaque, but there are some limitations to this study. First, despite our intention to obtain a precise and consistent measurement site by using the trigeminal ganglion as a prominent landmark, some discrepancies on follow-up imaging could not be entirely ruled out due to small size vessel and tortuosity. Second, only a focal site of BA plaque was measured, and it might not be representative of the changes in lesion burden for the rest of the vessel. 3D MR imaging measurements of the BA wall volume may overcome both of these limitations. Third, the appearance of a plaque component on different MR imaging sequences was not analyzed in our current studies and should be further investigated in the future.

Conclusions
Measurement of the WA in an atherosclerotic BA at the level of the trigeminal ganglion by HRMRI is reproducible. This imaging technique can potentially be used in future studies to monitor the effects of therapeutic intervention on BA plaque progression or regression.