Functional Connectivity MR Imaging of the Language Network in Patients with Drug-Resistant Epilepsy

BACKGROUND AND PURPOSE: Subtle linguistic dysfunction and reorganization of the language network were described in patients with epilepsy, suggesting the occurrence of plasticity changes. We used resting state FC-MRI to investigate the effects induced by chronic epilepsy on the connectivity of the language-related brain regions and correlated it with language performance. MATERIALS AND METHODS: FC-MRI was evaluated in 22 right-handed patients with drug-resistant epilepsy (11 with LE and 11 with RE) and in 12 healthy volunteers. Neuropsychological assessment of verbal IQ was performed. Patients and controls underwent BOLD fMRI with a verb-generation task, and language function was lateralized by an LI. Intrinsic activity fluctuations for FC analysis were extracted from data collected during the task. Six seeding cortical regions for speech in both hemispheres were selected to obtain a measure of the connectivity pattern among the language networks. RESULTS: Patients with LE presented atypical language lateralization and an overall reduced connectivity of the language network with respect to controls. In patients with both LE and RE, the mean FC was significantly reduced within the left (dominant) hemisphere and between the 2 hemispheres. In patients with LE, there was a positive correlation between verbal IQ scores and the left intrahemispheric FC. CONCLUSIONS: In patients with intractable epilepsy, FC-MRI revealed an overall reduction and reorganization of the connectivity pattern within the language network. FC was reduced in the left hemisphere regardless of the epileptogenic focus side and was positively correlated with linguistic performance only in patients with LE.

M R imaging plays a crucial role in the preoperative assessment of patients with intractable epilepsy. Structural MR imaging is routinely used for the detection of epileptogenic brain lesions, whereas fMRI localizes brain functions on the cerebral cortex and establishes their spatial relationship with lesions. One of the most frequent applications of fMRI is presurgical mapping of language functions and identification of the dominant hemisphere for speech. 1,2 Subtle linguistic dysfunction affects up to one-third of adults with chronic epilepsy, [3][4][5] even in the absence of a direct injury to the brain language network. Functional neuroimag-ing has demonstrated different patterns of activation and reorganization of the language network in patients with epilepsy compared with healthy subjects. Using fMRI, Brázdil et al 6 described a different activation pattern of the Broca area in a group of patients with left mesial-temporal epilepsy. In addition, task-evoked BOLD activity within the language network was found to be less unbalanced toward the dominant hemisphere in patients with epilepsy, compared with the healthy controls, especially in patients with early seizure onset. 2 Taken as a whole, these results suggest the possibility that epilepsy may induce functional changes across remote cortical regions, including those specialized for speech.
In addition to task-evoked fMRI studies, resting state FC-MRI can reveal the cortical connectivity among language-network regions by evaluating correlations of spontaneous BOLD signal-intensity fluctuations. 7-10 FC-MRI has attracted growing interest and new applications and can be reasonably considered a marker of neural connectivity. 11 Most important, FC-MRI seems particularly suited for clinical populations because subjects lie in the scanner without any imposed task, thus eliminating the need for complying with task instruc-tions. 12 Similarly, FC analysis of intrinsic brain activity, typically performed on resting state data, can be performed on task-related data by subtracting the contribution of the BOLD activation response. 12,13 Recently, Waites et al 14 found a decreased resting state FC-MRI connectivity among language regions and an increased connectivity between the left inferior frontal gyrus and part of the DMN in a group of patients with left temporal lobe epilepsy compared with controls. These observations further confirm the influence of epilepsy on language-network activity and possibly account for subtle language impairments that may affect patients with epilepsy. However, we have little information about potential hemispheric differences in these patients; and most important, the changes in FC associated with verbal performance have not been studied previously, to our knowledge.
In the present retrospective analysis, we investigated the FC pattern among language-network nodes in a group of patients (n ϭ 22) with either left-or right-lateralized drug-resistant epilepsy and in a group of matched healthy subjects (n ϭ 12). First, we evaluated the pattern of cortical activation during a conventional block-designed verb-generation task assessing the degree of language function lateralization across hemispheres. We then used FC-MRI to investigate the pattern of functional connectivity among 6 regions of the language network in the 2 hemispheres. Finally, we investigated the correlation between the pattern of FC-MRI and the neuropsychological evaluation of language performance.

Subjects
All the participants gave written informed consent before enrollment in the study, which was approved by our local ethics committee. Twenty-two right-handed patients (10 males, 12 females) with drugresistant epilepsy underwent an fMRI presurgical assessment to localize and lateralize language functions. Patient ages ranged from 16 to 51 years (mean, 29.8 Ϯ 10.0 years). Presurgical diagnostic protocol was the following: clinical history, neurologic examination, videoelectroencephalography monitoring, MR imaging, ictal and interictal single-photon emission CT, and neuropsychological assessment. The localization of the epileptogenic zone was based on the concordance of the electroclinical correlation with interictal positron-emission tomography data and MR imaging and neuropsychological changes, when present. According to the side of the epileptogenic focus, patients were divided in 2 subgroups: LE (n ϭ 11) and RE (n ϭ 11). The epileptogenic focus was detected in the temporal lobe in 16/22 (73%) patients and in the frontal lobe in 6/22 (27%). Epilepsy was cryptogenetic in 4 (18%) patients, whereas it was secondary to a cortical malformation in 3/22 (14%), vascular malformation in 2/22 (9%), DNET in 3/22 (14%), and MTS in 10/22 (45%) patients (Table 1). Large brain lesions causing epilepsy were not included in the study due to their tendency to destroy the normal anatomy of the brain and to directly affect (via contact or infiltration) the cortical regions for speech. Only patients with normal findings on MR imaging examinations of the brain or with small and no growing lesions distant from cortical regions for speech were included. Patient mean age at seizure onset was 16 years (range, 0.5-30 years).
The control group included 12 healthy right-handed subjects (mean age, 35 years; range, 25-44 years) presenting with normal neu-rologic examination findings, hearing, and sight. None of the controls experienced epilepsy during their lives. Right-handedness was determined by the Italian standardized version of the Edinburgh Handedness Inventory with an LI of Ͼ80. 15,16 The Wechsler Adult Intelligence Scale IV-Revised was administered in 17 of the 22 patients (9 LE and 8 RE) by an expert neuropsychologist to measure verbal IQ.

fMRI Task and Imaging Acquisition
Patients and controls underwent BOLD fMRI to localize cortical regions involved in language functions and to provide a measure of language hemispheric lateralization. Each subject was asked to silently perform an orthographically cued, block-designed, lexical verb-generation task, in which 4 task periods (30 seconds each) were intermixed with 5 rest periods (20 seconds each). During each task period, subjects were required to think for 2 seconds of pronouncing Ն1 verb associated with a noun, which was presented at the center of the screen for 1 second. During rest periods, subjects were instructed to relax while maintaining central fixation. While this task does not provide a measure of performance, it is nonetheless widely used in the clinical setting for its proved efficacy 17 and for not producing motion artifacts related to overt speech. Before scanning, all subjects executed a brief overt version of the task to ensure correct performance.
Visual stimuli were presented by using E-Prime software, Version 1.1 (Psychology Software Tools, Pittsburgh, Pennsylvania), projected via an LCD projector and viewed with a mirror placed above the subject's head.
Data were acquired on a 1.5T system (Magnetom Vision, Siemens, Erlangen, Germany; or Achieva, Philips Medical Systems, Best, the Netherlands). BOLD contrast functional images were acquired by using a T2*-weighted echo-planar free induction decay sequence (TR, 2000 ms; TE, 60 ms; matrix size, 64 ϫ 64; FOV, 256 mm; in-plane voxel size, 4 ϫ 4 mm; flip angle, 90°; section thickness, 4 mm; and no gap). The scan volume was oriented parallel to the anterior/posterior commissure plane and was acquired in ascending order covering the low and intermediate hemispheric convexity, including the classic

Activation fMRI Analysis
BrainVoyager QX, Version1.9 (Brain Innovation BV, Maastricht, the Netherlands) was used for image preprocessing and statistical analysis. Functional image time-series was first corrected for the differences in section-acquisition times, detrended, realigned with the structural images, and warped into standard Talairach anatomic space. 18 To assess the potential confound represented by group differences in the amount of motion artifacts, we quantified individual head shifts along the 3 rotation and translation anatomic axes. Statistical comparison across groups (patients versus controls) was performed by using a 2-tailed Student t test.
The fMRI statistical activation maps were generated according to a classic hypothesis-driven GLM method, combined with ICA, for the data-driven detection of the brain-activation response. Because the ICA approach does not rely on a predefined model of brain response, it is less influenced by modifications of BOLD activity induced by either disease or nonconformity of task execution. 19 The BOLD time series was decomposed into a set of spatially independent ICs 20 by means of the FastICA algorithm. 21 Each fMRI IC map was scaled to z scores 20 and was thresholded at z Ͼ 1.5 to display IC active voxels. 22 After the exclusion of artifactual ICs based on the IC fingerprint method, 23 we selected the IC showing the largest correlation coefficient between the predictor and respective time course, to obtain a data-driven estimate of the subject's task-related activation. Next, we created a mask by using the fMRI IC activations, and performed the standard GLM analysis on the masked fMRI data by using the IC time course as the predictor. This procedure generated statistical maps in z scores.
For each subject, the LI was calculated by counting the number of active voxels in the left and right hemispheres by using the following formula: LI ϭ (Vl Ϫ Vr) / (Vl ϩ Vr). 24,25 By definition, LI values range between Ϫ1 and ϩ1, where LI Ͼ ϩ0.20 indicates left lateralization; ϽϪ0.20, right lateralization; and ϩ0.20 and Ϫ0.20 inclusive, bilateral or not lateralized. Because LI varies with the statistical threshold, we calculated the MLI values obtained at multiple thresholds between P Ͻ .164 and the least stringent P value, according to which either LI ϭ Ϯ1, or no activation was detected. 24 The MLI value of each subject was used for statistical comparisons. To exclude voxels not specifically related to language function, we repeated the analysis, limiting the voxel count within the 4 regions of interest (centered on anterior and posterior language areas of each hemisphere) obtained by grouping the task-evoked BOLD activation of controls and patients, thresholded at P Ͻ .001. LI was computed by summing the active voxels of anterior and posterior language regions of each hemisphere.

FC Analysis
Regions of interest were independently identified on the basis of previous studies [26][27][28][29] to allow an unbiased intergroup comparison of FC-MRI. Three regions of interest (6-mm radius) were defined on the left hemisphere corresponding to the following: the pars triangularis of the IFG, Talairach coordinates: x ϭ Ϫ49, y ϭ ϩ17, z ϭ ϩ5; T2 mL: x ϭ Ϫ55, y ϭ Ϫ35, z ϭ ϩ3; and T1p: x ϭ Ϫ55, y ϭ Ϫ36, z ϭ ϩ24). The corresponding homologous coordinates were used to define re-gions of interest on the contralateral hemisphere, and the resulting 6 regions of interest were used in subsequent FC-MRI analysis.
Using a conjunction analysis, 9 we verified that the coordinates of the regions of interest were included in the fMRI activation maps that were observed in our subjects during the execution of the language task. With this method, group-level statistical maps obtained from the GLM analysis of the verb-generation task time course were derived by a random-effect analysis (P Ͻ .01, corrected with the FDR). 30 Before FC analysis, fMRI time series were further preprocessed, according to the procedures described by He et al 12 and Fair et al. 13 The preprocessing steps included the following: 1) band-pass filtering between 0.009 and 0.08 Hz, 2) regression of white matter and ventricle signals and their first derivatives, and 3) regression of 3D motion parameters and their first derivatives. In addition, to remove the contribution of the BOLD activation response from the signal-intensity time series, we performed a regression of the deterministic taskevoked components, by including a regressor corresponding to the verb-generation periods of the paradigm. Therefore, the FC-MRI analysis was performed over the entire residual dataset. While we cannot be sure that this procedure completely removed all the taskrelated components of the BOLD signal intensity, the resting state data obtained from block-designed fMRI studies, such as in our case, were reported to be well-suited for resting state FC-MRI analyses. 13 A GLM analysis on the residual dataset was performed in controls to ensure that no residual BOLD task-evoked activity was present in language-network regions. The resulting BOLD signal-intensity time course was averaged across all voxels of each region of interest. We calculated the Pearson correlation coefficients (r) between the signalintensity time course of each region of interest pair (pair-wise correlation) and between each region of interest and every voxel of the acquired volume (voxel-wise correlation maps).

Statistical Analysis
To obtain an approximately normal distribution of the FC values and thus apply parametric statistics, we converted the FC correlation r coefficients into z scores by means of the Fisher r-to-z transformation. 31 mFC values were estimated among all the seed regions (overall FC), within (intrahemispheric), and between (interhemispheric) hemispheres, by calculating respective correlation coefficients, according to the method proposed by Carter et al. 32 Group-level comparisons among mFC scores were performed by using analysis of variance and independent-sample t tests.
The correlation between mFC results and MLIs in patients and controls and the correlation between mFC results and verbal IQ scores in patients were estimated by using Pearson r coefficients because the mFC values, MLIs, and verbal IQ scores had an approximate normal distribution.

Control Analyses
Statistical comparison between the amount of head movement (mean shifts along rotation and translation axes) of patients and control groups did not yield significant differences (P Ͼ .05, 2-sample t test). At a threshold of P Ͻ .001, no residual task-evoked BOLD activity was present in languagenetwork regions of the control group.

Task-Dependent Activations and MLIs
In both control and patient groups, ICA-GLM analysis of BOLD data for the verb-generation task showed statistically significant activations (random effect analysis, P Ͻ .01 corrected with FDR) of the main language-related regions, including the left IFG, T2 mL, and T1p and their contralateral homologues (Fig 1A, -B). The combined conjunction map of patients and controls confirmed the common activation of language-related regions, including the predefined seed regions for subsequent FC analysis (Fig 1C).

FC of the Language Network
We analyzed the pattern of FC in language networks by using 3 cortical regions involved in language functions and their corresponding homologous coordinates of the controlateral hemisphere (see "Materials and Methods" section). Diagrams and cross-correlation matrices representing FC for control and patient groups are shown in Fig 2 (left panel). Healthy subjects showed significant connectivity among all of the language-network nodes, except for the L-T2 mL to R-T1p link (14 of 15 possible links among the 6 nodes); the strongest correlations were seen between the 2 IFGs and between the R-IFG and L-T1p nodes (r Ͼ 0.6). In the patient group (n ϭ 22), only 6 of 15 FC links were significant, including interhomologous connections and IFG-T2 mL links in both hemispheres.
To identify the most weakened functional connections, we performed a group comparison of the FC strength across region-of-interest pairs (2-tailed t tests). Significantly (P Ͻ .01) reduced FC values are indicated by black lines in Fig 2 (right  panel), which illustrate that the most affected node is L-T1p. Overall, these results demonstrate that the strong FC among the language network in healthy subjects was significantly weakened in patients with epilepsy, especially in the L-T1p node.
We tested possible effects of the epileptogenic focus side on the language-network connectivity by splitting the group of patients according to the epileptogenic focus side (LE or RE) Fig 1. A and B, Verb-generation task statistical activations from group-level ICA-GLM analysis in the control (n ϭ 12) and patient (n ϭ 22) groups, respectively (P Ͻ .01, FDR-corrected for multiple comparisons; only clusters Ͼ10 mm 2 are represented). C, Predefined seed regions of interest for the FC analysis are superimposed on the conjunction plots, displaying common activated voxels in controls and in patients in both LE and RE subgroups. All seed centers were found to be included within the activated shared voxels. (Fig 3). Both subgroups presented several missing FC links, both within and between hemispheres (P Ͻ .01). Regardless of epileptogenic focus side, 2 significant language-network links were present in the right hemisphere, whereas only a single  significant link was present in the left hemisphere. The inter-T1p and inter-IFG-T2 mL links were preserved bilaterally in patients with both LE and RE.
Next, we compared the pattern of FC between controls and each of the patient subgroups, estimating the mFC within and between hemispheres. The results are presented in Table 2. Regardless of the epileptogenic focus side, the overall, inter-, and left-intra mFCs were significantly reduced in both subgroups, whereas the right intrahemispheric mFC did not differ compared with controls. Thus, epilepsy seems to generally affect the normal FC pattern, by particularly disrupting language-network connectivity within the left (dominant) hemisphere, regardless of epileptogenic focus side.

Voxel-Wise FC Maps
We also analyzed the spatial pattern of widespread cortical connectivity by using each language-network region as a seed for the creation of group-level FC statistical voxel-wise maps. Compared with the regional analysis, these maps provided a test of the spatial specificity of the correlation among the language network and could potentially reveal connectivity with other cortical regions. Figure 4 illustrates the connectivity map generated by using the L-T1p as a seed, a region that was found to be particularly compromised in regional analysis. First, the map of the control group demonstrated that the seed showed correlation almost exclusively with other regions belonging to the language network, indicating a high degree of spatial specificity. Furthermore, in accordance with the regional analysis, voxel-wise maps of the group of patients confirmed a significant reduction of connectivity between L-T1p and other language-network regions, compared with healthy subjects. Most interesting, L-T1p was functionally connected with the PCC only in the patient group (white circles in Fig 4). This finding suggested that, besides the disruption of several FC links within the language network, patients may establish peculiar connections with other brain regions that are not only unrelated to language functions but are even part of a cortical network that typically shows deactivation during the execution of externally oriented tasks (the so-called default mode network 33 ).

Correlation with Clinical Data
We investigated the relationship between the FC within the language network and patient performance at language tasks. The mean verbal IQ score of patients was 79.6; there was no significant difference between patients with LE (76.7) and RE (82.7) (P ϭ .54, 2-tailed t test). Because we found a significant reduction of the intrahemispheric mFC only in the left hemisphere, regardless of the epileptogenic focus side, we evaluated the correlation between the amount of left intrahemispheric connectivity and verbal IQ scores. The verbal IQ scores significantly correlated with the L-Intra mFC in patients with LE (r ϭ 0.7, P Ͻ .05) but not in patients with RE (r ϭ 0.05; P ϭ .44). There was no correlation between verbal IQ and the right intrahemispheric mFC for either subgroup. We did not find a significant correlation between verbal IQ performance and LIs. Thus, while patients with both LE and RE exhibited compromised connectivity within language-network nodes of the left hemisphere compared with controls, the degree of connectivity was related to language performance exclusively in patients with LE.

Discussion
Mild linguistic impairments, often occurring in chronic drugresistant epilepsy, are not associated with evident changes in structural neuroimaging. Previous task-evoked fMRI 2,6,34 and magnetoencephalography 35 studies found aberrant localization of language functions in selected groups of patients with epilepsy. As a complementary procedure to the analysis of task-evoked brain activity, FC-MRI can evaluate the integrity of the entire network supporting specific functions during resting state. In patients with left temporal lobe epilepsy, Waites et al 14 described the disruption of several FC links between otherwise normally task-activated brain regions; however, they only evaluated patients with left MTS. Furthermore, the study did not include left temporal regions, which are crucially related to semantic language processing, 29,36 nor homologous regions of the right hemisphere, among the FC seeds.
In the present study, FC was estimated in 12 healthy subjects and 22 patients with either LE or RE among a network including 3 homologous cortical regions of both hemispheres. In addition, we investigated potential correlations between the  strength of FC among the language networks and language performance.

Intersubject Comparison
There are multiple potential limitations when comparing task-evoked BOLD activity between groups of patients and controls. The main issue is that a difference in task performance, rather than a neurologic condition, may explain a difference in the BOLD task-evoked response. A variation in the way patients, compared with controls, cooperate with task instructions, especially when they continuously receive antiepileptic medications that may affect general arousal, has already been considered in previous studies. 14,37 Crucially, the FC low-frequency fluctuations of the BOLD signal intensity during the resting state do not depend on task execution 7 and are, therefore, less influenced by patient attentive capability and cognitive performance. 12 In the present study, we extracted the intrinsic signal-intensity fluctuations from a conventional verb-generation block paradigm, regressing out the deterministic components from the BOLD time course. 12,13 We also investigated the presence of group differences in terms of head motion during imaging acquisition because patients may have exhibited more movement artifacts. The quantity of head translational and rotational movements did not differ between patients and controls. Finally, we tried to preserve the intersubject comparability by only including either patients without lesions or with lesions that did not broadly alter the normal cortical anatomy.

Task-Dependent Language Activations
In both healthy subjects and patients, task-evoked BOLD activity was observed in anterior and posterior brain regions classically associated with language functions, 38 with a larger recruitment of right cortical areas in patients with epilepsy compared with controls. This was not unexpected because previous fMRI studies 2,34,39 found a significant correlation between epilepsy and atypical language lateralization, especially in patients with early-onset epilepsy. Consistent with several of these reports, 34,39 we found a significant difference in the MLIs between patients with LE and those with RE: Patients with LE had lower MLIs with respect to either controls or patients with RE, confirming a selective atypical lateralization.

FC of the Language Network
We tested the amount of FC of the language network across both hemispheres for 2 main reasons: Language reorganization in patients with epilepsy involves both hemispheres, and language activations also spread through the nondominant hemisphere in healthy subjects. 40 We, therefore, chose 3 classical cortical regions related to semantic and phonologic language processing in the dominant hemisphere, [26][27][28][29] together with their contralateral homologues. These areas represent the essential brain regions for speech known to be functionally and structurally connected. [41][42][43] Most interesting, the control group exhibited a high degree of interhemispheric connectivity while showing a left-hemisphere language lateralization, supporting a difference between task-evoked BOLD activity and the underlying pattern of intrinsic FC.
Patients with epilepsy generally showed a reduced pattern of connectivity across the language network at the same statis-tical threshold, in accordance with previous studies, 14,42 demonstrating a reduction of FC between language areas. In the present work, we could further observe a reduction of several links both within and between hemispheres (Fig 2). When we compared the FC strength between patients and controls (Fig  2, right panel), the left T1p was found to be the most weakened node within the language network (4 significantly reduced links). Remarkably, Alstott et al, 44 by using a computational model from diffusivity-based tractography data, described dramatic effects on brain network connectivity when simulating a lesion that disrupted the region of the temporoparietal junction. Although these results come from radically different experimental environments, they bolster the crucial role of the posterior temporal region for functional network integrity and also suggest a particular vulnerability of this cortical area to nonlocal disturbances over the connectivity network.
The significant reduction of connectivity in patients compared with controls should not be considered a marker of a generalized reduction of cortical connectivity due to epilepsy. As a matter of fact, the voxel-wise analysis demonstrated that patients also exhibit increased connectivity between the L-T1p node and the PCC, replicating the results of Waites et al, 14 who found greater resting state connectivity between another region of the language network, the left IFG, and the PCC in patients with left temporal lobe epilepsy compared with controls. The PCC is part of the DMN, defined as the set of cortical regions that are consistently deactivated during externally oriented task execution. 33,45 Most interesting, the DMN also exhibits a negative correlation with "antagonist" attentive brain networks, both at rest 9 and during task execution, 46 suggesting that the negative correlation between the DMN and task-positive networks is a marker of an efficient cortical organization. In our patients, the presence of a diminished resting state connectivity among the language networks, coupled with an increased connectivity between the language network and the DMN, may be part of plasticity changes induced by epilepsy. 47 Whether this correlation is part of a compensatory mechanism to establish new functional pathways replacing the damaged ones or if it represents the unsuppressed interference from DMN regions with the language network is a matter for further investigation. Recently, the degree of negative correlation between DMN and attentive networks was found to be significantly related to an individual's task performance. 46 We can, therefore, hypothesize that the presence of a T1p-PCC positive correlation in our patients underlies unfavorable dysfunctional mechanisms.
When we investigated the effect of the epileptogenic focus side on the FC pattern by separating the group of patients into LE and RE subgroups, we found that the intrahemispheric mFC was always significantly reduced in the left (dominant) hemisphere regardless the epileptogenic focus side (Table 1 and Fig 3). This finding was unpredicted because some significant epileptogenic focus effects on the ipsilateral FC could be hypothesized when considering the anatomic proximity to the focus. Mechanisms other than proximity justify the presence of a language-network-wise effect of epilepsy 48 : 1) Interictal and ictal discharges may propagate from the seizure focus to other brain regions through existing fiber tracts 49 ; 2) the postulated attempt of the brain to inhibit epileptic seizures by upregulating inhibitory functions may have the side effect of suppressing neuronal activity, including the language system 50 ; and 3) antiepileptic drugs may alter the physiologic neurotransmission, causing changes in the resting state FC. 51

BOLD-Neuropsychological Correlations
We additionally investigated the presence of potential correlations between the pattern of FC and the neuropsychological assessment in patients with LE and RE. Although the reduction of FC across the language network seems to largely exceed the amount of speech impairment, because patient scores were within the normal range at the group level, it may, nonetheless, be more subtly related to language performance and explain part of the interindividual variability of the neuropsychological assessment.
What is crucial, despite both patient subgroups showing decreased intrahemispheric connectivity within the left hemisphere, only patients with LE exhibited a significant correlation between left hemispheric mFC and verbal IQ scores. This result has 2 important implications: First, the reduction of left intrahemispheric connectivity in patients with LE is related to language performance. Second, the reduction of left intrahemispheric connectivity per se does not account for language performance because no correlation was observed in patients with RE. We offer an explanation of this result by simultaneously considering both the lateralization index, which is a measure of the task-evoked hemisphere dominance, and the pattern of FC. In fact, only the patients with LE exhibited atypical language lateralization compared with controls (mean MLI, 0.11). We reasoned that verbal performance is explained only when the reduction of the left intrahemispheric FC is observed simultaneously with an atypical language lateralization pattern.

Conclusions
The 2 subgroups of patients with LE and RE showed a selective reduction of left intrahemispheric FC of the language network, confirming both a local and distant influence of epileptogenic foci on cortical networks. The left intrahemispheric FC of the language network correlated with verbal performance only in patients with LE.
These results indicate the advantages of including the assessment of the FC, beyond the simple analysis of task-evoked activations, for a more comprehensive understanding of functional modifications induced by epilepsy. Larger study groups, together with postoperative follow-ups, are required to definitively assess the role of FC-MRI in the presurgical work-up of patients with intractable epilepsy.