@article {Yun, author = {T.J. Yun and H.R. Cho and S.H. Choi and H. Kim and J.-K. Won and S.-W. Park and J.-h. Kim and C.-H. Sohn and M.H. Han}, title = {Antiangiogenic Effect of Bevacizumab: Application of Arterial Spin-Labeling Perfusion MR Imaging in a Rat Glioblastoma Model}, year = {2016}, doi = {10.3174/ajnr.A4800}, publisher = {American Journal of Neuroradiology}, abstract = {BACKGROUND AND PURPOSE: The usefulness of arterial spin-labeling for the evaluation of the effect of the antiangiogenic therapy has not been elucidated. Our aim was to evaluate the antiangiogenic effect of bevacizumab in a rat glioblastoma model based on arterial spin-labeling perfusion MR imaging.MATERIALS AND METHODS: DSC and arterial spin-labeling perfusion MR imaging were performed by using a 9.4T MR imaging scanner in nude rats with glioblastoma. Rats were randomly assigned to the following 3 groups: control, 3-day treatment, and 10-day treatment after bevacizumab injection. One-way analysis of variance with a post hoc test was used to compare perfusion parameters (eg, normalized CBV and normalized CBF from DSC MR imaging and normalized CBF based on arterial spin-labeling) with microvessel area on histology. The Pearson correlations between perfusion parameters and microvessel area were also determined.RESULTS: All of the normalized CBV from DSC, normalized CBF from DSC, normalized CBF from arterial spin-labeling, and microvessel area values showed significant decrease after treatment (P \< .001, P \< .001, P = .005, and P \< .001, respectively). In addition, normalized CBV and normalized CBF from DSC and normalized CBF from arterial spin-labeling strongly correlated with microvessel area (correlation coefficient, r = 0.911, 0.869, and 0.860, respectively; P \< .001 for all).CONCLUSIONS: Normalized CBF based on arterial spin-labeling and normalized CBV and normalized CBF based on DSC have the potential for evaluating the effect of antiangiogenic therapy on glioblastomas treated with bevacizumab, with a strong correlation with microvessel area.AbbreviationsASLarterial spin-labelingGBMglioblastomaMVAmicrovessel areanCBFnormalized CBFnCBVnormalized CBVrCBFrelative CBFrCBVrelative CBV}, issn = {0195-6108}, URL = {https://www.ajnr.org/content/early/2016/05/12/ajnr.A4800}, eprint = {https://www.ajnr.org/content/early/2016/05/12/ajnr.A4800.full.pdf}, journal = {American Journal of Neuroradiology} }