PT  - JOURNAL ARTICLE
AU  - R. Jain
AU  - J. Gutierrez
AU  - J. Narang
AU  - L. Scarpace
AU  - L.R. Schultz
AU  - N. Lemke
AU  - S.C. Patel
AU  - T. Mikkelsen
AU  - J.P. Rock
TI  - In Vivo Correlation of Tumor Blood Volume and Permeability with Histologic and Molecular Angiogenic Markers in Gliomas
AID  - 10.3174/ajnr.A2280
DP  - 2011 Feb 01
TA  - American Journal of Neuroradiology
PG  - 388--394
VI  - 32
IP  - 2
4099  - http://www.ajnr.org/content/32/2/388.short
4100  - http://www.ajnr.org/content/32/2/388.full
SO  - Am. J. Neuroradiol.2011 Feb 01; 32
AB  - BACKGROUND AND PURPOSE: Tumor angiogenesis is very heterogeneous and in vivo correlation of perfusion imaging parameters with angiogenic markers can help in better understanding the role of perfusion imaging as an imaging biomarker. The purpose of this study was to correlate PCT parameters such as CBV and PS with histologic and molecular angiogenic markers in gliomas. MATERIALS AND METHODS: Thirty-six image-guided biopsy specimens in 23 patients with treatment-naive gliomas underwent PCT examinations. We correlated MVD, MVCP, VEGFR-2 expression, tumor cellularity, and WHO grade of the image-guided biopsy specimens with the PCT parameters. Histologic sections were stained with hematoxylin-eosin, CD34, and VEGFR-2 and examined under a light microscope. These histologic and molecular angiogenic markers were correlated with perfusion parameters of the region of interest corresponding to the biopsy specimen. Pearson correlation coefficients and multiple regression analyses by using clustering methods were performed to assess these correlations. RESULTS: CBV showed a significant positive correlation with MVD (r = 0.596, P < .001), whereas PS showed a significant positive correlation with MVCP (r = 0.546, P = .001). Both CBV (r = 0.373, P = .031) and PS (r = 0.452, P = .039) also showed a significant correlation with WHO grade. VEGFR-2 positive specimens showed higher PS and CBV; however, neither was statistically significant at the .05 level. CONCLUSIONS: CBV showed a significant positive correlation with MVD, whereas PS showed a significant positive correlation with MVCP, suggesting that these 2 perfusion parameters represent different aspects of tumor vessels; hence, in vivo evaluation of these could be important in a better understanding of tumor angiogenesis. CBFcerebral blood flowCBVcerebral blood volumeFlk-1fetal liver kinase-1Flk-2fetal liver kinase-2HIPAAHealth Insurance Portability and Accountability ActKtransvolume transfer coefficientMVCPmicrovascular cellular proliferationMVDmicrovascular densityMTTmean transit timePCTperfusion CTPSpermeability surface area productrCBVrelative CBVSEstandard errorTVAtotal microvascular areaVEGFvascular endothelial growth factorVEGFR-2vascular endothelial growth factor receptor-2WHOWorld Health Organization