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Clinical, histological, and immunohistochemical features predicting 1p/19q loss of heterozygosity in oligodendroglial tumors

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Abstract

To identify a better diagnostic criteria for oligodendroglial tumors, we investigated the clinical, histological, and immunohistochemical features that would be able to predict a 1p/19q loss of heterozygosity (LOH) in these tumors. We performed a PCR-based LOH test with the 1p and 19q microsatellite markers by microdissecting tumor in 56 samples (44 oligodendrogliomas and 12 mixed oligoastrocytomas) of paraffin-embedded tissue. Patients with oligodendroglial tumors with 1p/19q LOH had a statistically significant better prognosis for overall survival. Comparative analysis of several features indicated that the 1p/19q LOH tumors were associated with two histological features, “tumor cellularity” and “perinuclear halo,” and low O6-methylguanine-DNA-methyltransferase (MGMT) expression and high cytoplasmic glutathione S-transferase pi (GST-π) expression. In addition, the incidence of 1p/19q LOH was infrequent in the youngest age category (less than 20 years old) studied. Using the new features, we could predict the 1p/19q status of oligodendroglial tumors with greater than 90% accuracy. Therefore, applying these features in clinical practice, would be helpful in clarifying oligodendroglial tumor diagnosis.

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Acknowledgements

This work was supported by the Yonsei University Research Fund of 2003. The authors appreciate the kind gift of Musashi-1 monoclonal antibody and Olig 2-C monoclonal antibody from Dr. Hideyuki Okano (Department of Physiology, Keio University, Tokyo, Japan) and Dr. Hideaki Yokoo (Department of Pathology, Gunma University Graduate School of Medicine, Gunma, Japan), respectively. We also thank Ms. Soohyun Jeong, Mr. Sang Pil Ahn, Mr. Hyeong Jae Jeong and Mr. Kyeong-il Kim for their technical assistance.

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  1. Department of Pathology, College of Medicine, Yonsei University, Seoul, 120–752, Korea

    Se Hoon Kim, Hoguen Kim & Tai Seung Kim

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  1. Se Hoon Kim
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Correspondence to Tai Seung Kim.

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Kim, S.H., Kim, H. & Kim, T.S. Clinical, histological, and immunohistochemical features predicting 1p/19q loss of heterozygosity in oligodendroglial tumors. Acta Neuropathol 110, 27–38 (2005). https://doi.org/10.1007/s00401-005-1020-x

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  • DOI: https://doi.org/10.1007/s00401-005-1020-x

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