Intracellular diffusion of water

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Abstract

Self-diffusion of cell water has been measured at diffusion times ranging from 0.3 ms to 1.0 s for human red cells, yeast, and brine shrimp using various pulsed gradient NMR methods. Intracellular diffusion coefficients and membrane permeabilities are calculated from these data with the aid of previous theoretical results for regularly spaced permeable planar barriers. The intracellular diffusion coefficients of water range from 1.2 × 10−6 to 6 × 10−6 cm2/s for the various samples. Outer-membrane permeabilities to water range from 0.0001 to 0.01 cm/s. The self-diffusion coefficient of lipid in a sample of human breast adipose tissue was found to be 1.5 sx 10−7 cm2/s.

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    The experimental portions of this work have been reported in Technical Report No. NWSC/CR/RDTR-42, Naval Weapons Support Center, Crane, Indiana, October 1976, available from Defense Documentation Center Cameron Station, Alexandria, Virginia, AD-A031357; in the meeting of the American Physical Society held 29 March–1 April 1976 in Atlanta, Georgia, paper FF5; in Magnetic Resonance in Colloid and Interface Science (Resing, H. A., and Wade, C. G., eds.), ACS Symposium Series No. 34 (1976), pp. 16–30, American Chemical Society, Washington, D. C.; in the 21st Annual Biophysical Society meeting, 15–18 February 1977 in New Orleans, paper F-PM-B11; and in the 24th Annual Biophysical Society meeting, 1–5 June 1980 in New Orleans, paper 813.

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    Present address: Exxon Nuclear Idaho Co., Idaho Falls, Idaho 83401.

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