Chapter 42 Fluorescent Cell Labeling for in Vivo and in Vitro Cell Tracking
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2021, iScienceCitation Excerpt :To determine the long-term proliferative capacity of these non-cycling cells, we labeled cells with the lipophilic fluorescent dye PKH26. Unlike CM-DiL, which is suitable for short-term label retention studies, PKH26 labeling can be used to identify non-cycling cells for up to 6 months (in vitro and in vivo) (Cicalese et al., 2009; Horan et al., 1990). PDOs were dissociated to single cells, labeled with PKH26, and replated in Matrigel culture.
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2018, iScienceCitation Excerpt :This result suggests that the hydrophilicity of a molecule plays a significant role in the plasma membrane localization of dye. It is well established that for plasma membrane localization, the dyes should be lipophilic and longer hydrophobic alkyl chains ensure irreversible localization (Betz et al., 1996; Horan et al., 1990); however, the role of hydrophilicity has not been thoroughly investigated. Previously, it has been observed that the dicationic version of the amphiphilic dye, ANNINE-6plus, ensures better plasma membrane binding than the zwitterionic version, ANNINE-6, but the importance of hydrophilic head groups of amphiphilic dyes in plasma membrane binding was not studied (Fromherz et al., 2008).
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