Elsevier

Clinical Neurophysiology

Volume 116, Issue 10, October 2005, Pages 2381-2391
Clinical Neurophysiology

Reproducibility of measures of neurophysiological activity in Wernicke's area: A magnetic source imaging study

https://doi.org/10.1016/j.clinph.2005.06.019Get rights and content

Abstract

Objective

The purpose of this study was to evaluate the reproducibility of estimates of neurophysiological activity obtained with Magnetic Source Imaging.

Methods

Split-half data sets were obtained from 14 healthy volunteers during performance of a continuous recognition task for spoken words. The concurrent validity of spatiotemporal activation maps obtained with this task has been previously verified through comparisons with the Wada test and electrocortical stimulation mapping. Consecutive late activity sources (>200 ms after stimulus onset) were modeled independently as equivalent current dipoles (ECDs) and used to identify the location of language-specific cortex in the left hemisphere (Wernicke's area).

Results

Linear displacement of the geometric center of the cluster of ECDs in this region ranged between 2 and 8 mm across subjects. Intraparticipant variability (range) in the onset latency of activity was ±50 ms, while the range of change in global field power for the entire set of ECDs in Wernicke's area was less than 17% in all cases.

Conclusions

The results indicate that despite its many conceptual limitations the ECD model can provide reliable estimates of regional cortical activity associated with the engagement of linguistic processes.

Significance

The results highlight the need for reproducibility studies when research questions pose particular requirements for precision of estimates of regional neurophysiological activity.

Introduction

High-resolution, non-invasive studies of the neuroanatomical basis of language function have been made possible in the past decade with advances in imaging techniques. Reported findings have generally been consistent with classic language models (Wernicke, 1874) that were based largely on the study of linguistic deficits in patients with brain lesions (Damasio, 1998, Price, 2000) and the results of invasive brain mapping procedures (Lesser et al., 1994, Ojemann et al., 1989). An anterior left hemisphere speech region, classically defined as Broca's area and located in the posterior part of the inferior frontal gyrus, has been shown to be active during expressive language tasks. Activation in Wernicke's area, located in the posterior portion of the superior temporal gyrus in the left hemisphere, is found during receptive language tasks. Agreement between the outline of functional images and prior expectations regarding the outline of the brain mechanism responsible for language functions instigated several clinical trials of functional brain imaging methods as tools for the identification of eloquent cortex prior to surgical resection of tumors, epileptogenic foci, and vascular malformations (e.g. Binder et al., 1997, Stapleton et al., 1997, Bookheimer et al., 1997, Simos et al., 1999a, Castillo et al., 2001, Kober et al., 2001, Rutten et al., 2002).

Given the practical and technical limitations associated with invasive presurgical brain mapping procedures (e.g. Önal et al., 2003), particularly when evaluating pediatric patients, clinicians have welcomed the availability of non-invasive brain imaging alternatives (e.g. Forss et al., 2000, Pataraia et al., 2002). Whole-head magnetoencephalography (MEG) is the newest of functional brain imaging techniques (e.g. Hämäläinen et al., 1993, Kaufman and Ju, 2003), and only recently suitable activation protocols have been developed for identifying the location and extent of language specific cortex. Those centers employing MEG for the presurgical mapping of language functions have shown very favorable results in terms of both its reliability and validity. As would be expected, language-related activity sources have been extracted from the late components of event-related magnetic field records (ERFs), which occur later than 200 ms after the presentation of the stimulus (Halgren et al., 2002, Helenius et al., 2002, Kober et al., 2001, Simos et al., 1998, Simos et al., 1999a, Simos et al., 1999b).

A further prerequisite for the establishment of any brain imaging technique as clinically useful and equivalent to invasive techniques is that the random, intra-participant variability in source localization for selected activation protocols be substantially smaller than the spatial resolution limit of the gold-standard method which is in the order of 1 cm. While reproducibility studies have been successfully completed for early sensory (Baumann et al., 1990, Gallen et al., 1994, Pantev et al., 1991) and motor-related activity (Castillo et al., 2004), there is a scarcity of studies investigating the test–retest reliability of late components associated with language-specific neurophysiological operations (Breier et al., 2000).

In the present study, we examine the reproducibility of estimates of magnetic activity associated with the engagement of Wernicke's area in a simple word recognition task in healthy volunteers. The activation protocol used in this study is identical to the one used in previous clinical validation studies, and it is known to produce reliable magnetic activity sources in posterior temporal and temporoparietal regions in the left hemisphere, where Wernicke's area is located in the vast majority of neurologically intact right-handers. These sources represent cortical electrical activity that takes place during the late phases of stimulus processing (i.e. >200 ms after stimulus onset) and lasts for several hundred milliseconds. This activation protocol was, therefore, deemed suitable for studying the stability of estimates of language-specific, regional brain activity. Reproducibility measures were based on intrasession (split-half) variability, affected by short-term changes in state factors influencing regional neurophysiological activation (motivation, attention, fatigue, habituation, etc.), as well as by random changes in extraneous and biological magnetic noise.

Section snippets

Subjects

Participants were 14 right-handed healthy volunteers (seven men) between 24 and 52 years of age (mean: 33.4 years) without history of neurological or psychiatric disease, or hearing impairment. Hand preference was evaluated using the Edinburgh Handedness Inventory (Oldfield, 1971) with all subjects scoring +0.6 or greater. Prior to the study all participants gave their written informed consent, after the purpose of the study and the nature of the procedures involved had been explained to them.

Stimuli and task

Preliminary analyses

In every dataset magnetic flux during the 150 ms pre-stimulus interval, averaged across all magnetic sensors used to calculate the Wernicke's area ECDs (an estimate of background environmental and biological noise), was below 10 fT. Field strength for ECDs comprising each cluster ranged between 31 and 205 fT across participants (i.e. over three times higher than background noise), a level that is generally considered adequate for reliable computation of ECD source parameters. The vast majority of

Discussion

Previous studies investigating the test–retest reliability of MSI focused exclusively on records of early activity originating from modality-specific sensory (auditory or somatosensory) cortices (Gallen et al., 1994, Pantev et al., 1991). The present study is the first to our knowledge of the reproducibility of MEG-derived estimates of late activity in the context of a language task. Moreover, the activation task and data acquisition and analyses procedures used in the present study were

Acknowledgements

This study was supported in part by NINDS grant NS37941 and NICHD/OERI grant HD30885to Andrew C. Papanicolaou, and by the Vivian L. Smith Center for Neurologic Research, Houston, TX.

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