Comparison of different needle diameters and flow rates on bone marrow mononuclear stem cell viability: an ex vivo experimental study
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2019, Nervous System Drug Delivery: Principles and PracticeComparison of the methods evaluating post thawing viability of peripheral blood stem cell graft
2019, Transfusion and Apheresis ScienceCitation Excerpt :As far as we know, this study is the first study in the literature comparing the commonly used, pratique methods in clinical practice for assessing viability in PBSC graft and more sensitive flow cytometry-7AAD method. The studies in the literature comparing the methods assessing the viability of hematopoietic stem cells were conducted in the way of assessing the viability of bone marrow stem cells by exposing them to various in vitro injuring agents [15,16]. Mascotti et al. mixed fresh bone marrow cells with the bone marrow cells killed as a result of heat shock exposure at a certain ratio and compared the trypan blue and acridine orange/propidium iodide method in the bone marrow cells with known viability rate and evaluated the association of these methods with cell culture assays [16].
Processing of autologous bone marrow cells by apheresis technology for cell-based cardiovascular regeneration
2012, CytotherapyCitation Excerpt :Apart from the observed negative correlation between age and progenitor cell collection efficiency (Figure 2) (29), the primary ischemic disease and potential co-morbidities might modify the BM progenitor cell pool (30,31). Further possible reasons for the low collection efficiency include the effect of hemodilution and/or technical considerations, for example the kind of BM aspiration needle used (28,32). Following BM harvest, we enriched the BM-MNC fraction with a semi-automatic cell separator device.
Effect of volume flows on the viability of MSCs during injection through a cannula
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