Abstract
The following protocol is one of the longest-established methods of DNA extraction and works well with a wide range of solid tissues. Proteins are digested with proteinase K and extracted with phenol chloroform. DNA is then precipitated with ethanol. The resultant DNA (10–50 μg) is of high molecular weight and is a suitable template for long polymerase chain reaction (PCR).
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© 2003 Humana Press Inc.
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Pearson, H., Stirling, D. (2003). DNA Extraction from Tissue. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:33
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DOI: https://doi.org/10.1385/1-59259-384-4:33
Publisher Name: Humana Press
Print ISBN: 978-0-89603-642-0
Online ISBN: 978-1-59259-384-2
eBook Packages: Springer Protocols