The goal of neuro-oncologic surgery is to maximize tumor resection while preserving vital brain functions. Identification of the relation between the tumor and adjacent functional cortical areas as well as efferent subcortical white matter tracts is important for preservation of function. Combined diffusion tensor imaging with magnetic resonance (MR) tractography and functional blood oxygen level-dependent MR imaging were applied successfully for preoperative planning and guidance in 2 patients with tumors near the motor cortex. The combination of these novel functional imaging techniques can provide new information for presurgical planning.