Abstract
In vivo, volume-selected 1H NMR spectroscopy employing the SPACE technique was used to monitor biochemical changes in the thiamin deficient rat brain in response to glucose loading. The concentrations of brain N-acetylaspartate, glutamate/glutamine/gamma-aminobutyric acid, lactate and glucose differed significantly from those of control animals. The results are consistent with a metabolic block at the reaction catalyzed by the thiamin dependent enzyme alpha-keto glutarate dehydrogenase soon after the onset of neurological symptoms of thiamin deficiency, and a further block at pyruvate dehydrogenase arising late in the course of thiamin deficiency.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acidosis, Lactic / etiology
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Animals
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Aspartic Acid / analogs & derivatives
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Aspartic Acid / analysis
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Ataxia / etiology
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Brain Chemistry*
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Female
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Glucose / analysis
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Glucose / pharmacology*
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Glucose / toxicity
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Glutamates / analysis
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Glutamic Acid
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Glutamine / analysis
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Ketoglutarate Dehydrogenase Complex / metabolism
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Lactates / analysis
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Lactic Acid
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Magnetic Resonance Spectroscopy*
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Pyrithiamine / toxicity
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Pyruvate Dehydrogenase Complex / metabolism
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Rats
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Rats, Wistar
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Thiamine Deficiency / complications
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Thiamine Deficiency / metabolism*
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gamma-Aminobutyric Acid / analysis
Substances
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Glutamates
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Lactates
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Pyruvate Dehydrogenase Complex
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Glutamine
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Aspartic Acid
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Lactic Acid
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Glutamic Acid
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gamma-Aminobutyric Acid
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Pyrithiamine
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N-acetylaspartate
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Ketoglutarate Dehydrogenase Complex
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Glucose