The purpose of this study was, first, to evaluate correlations between four potential magnetic resonance (MR) parameters for severe tissue destruction in multiple sclerosis (MS) lesions. Second, to evaluate the effect of incidental magnetization transfer (MT) effect on hypointense lesions in multislice T1 spin echo (SE) imaging. In 49 lesions, from 10 MS patients, MT ratio (MTR), T1 relaxation time, signal intensity (SI) on T1-weighted SE images normalized to normal-appearing white matter (NAWM), and SI normalized to cerebrospinal fluid (CSF) were measured. Differences in contrast of hypointense lesions were measured between single slice and multislice imaging. MTR correlated significantly (p < .001) with longitudinal relaxation rates (1/T1) (r = 0.84), SI normalized to NAWM (r = 0.78), and SI normalized to CSF (r = 0.75). The degree of reduction in contrast, caused by multislice imaging, correlated significantly with MTR of lesions (r = 0.60, p < .001), leading to reduction of hypointense lesion load (p < .01). We conclude that all four MR markers for severe tissue destruction are highly correlated when applied to selected hypointense lesions on T1-weighted SE images. Due to "incidental" off-resonance excitation, contrast and hypointense lesion load will be reduced in multislice T1-weighted SE images.