Abstract
Object:
The goal of this study was to evaluate gene delivery to a benign brain tumor.
Methods:
A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.
Conclusions:
The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics
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Animals
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Coloring Agents
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DNA, Recombinant / genetics
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Dogs
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Escherichia coli / genetics
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Ethmoid Bone / pathology
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Female
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Galactosides
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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Genes, Reporter / genetics
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Genetic Therapy*
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Genetic Vectors
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Indoles
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Injections, Intra-Arterial
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Meningeal Neoplasms / blood supply
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Meningeal Neoplasms / pathology
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Meningeal Neoplasms / surgery
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Meningeal Neoplasms / therapy
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Meningeal Neoplasms / veterinary*
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Meningioma / blood supply
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Meningioma / pathology
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Meningioma / surgery
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Meningioma / therapy
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Meningioma / veterinary*
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Transduction, Genetic
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beta-Galactosidase / genetics
Substances
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Coloring Agents
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DNA, Recombinant
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Galactosides
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Indoles
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beta-Galactosidase
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5-bromo-4-chloro-3-indolyl beta-galactoside