A Standardized Method for Measuring Intracranial Arterial Stenosis

Equation for Measuring Intracranial Arterial Stenosis

The equation used for determining percent stenosis of a major intracranial artery was as follows: percent stenosis = [(1 − (D_stenosis/D_normal))] × 100, where D_stenosis = the diameter of the artery at the site of the most severe degree of stenosis and D_normal = the diameter of the proximal normal artery. D_normal was determined by the following criteria: for the middle cerebral, intracranial vertebral, and basilar arteries, the diameter of the proximal part of the artery at its widest, non-tortuous, normal segment was chosen (first choice). If the proximal artery was diseased (eg, middle cerebral artery origin stenosis), the diameter of the distal portion of the artery at its widest, parallel, non-tortuous normal segment was substituted (second choice). If the entire intracranial artery was diseased, the most distal, parallel, non-tortuous normal segment of the feeding artery was measured (third choice). For example, if the entire basilar artery was diseased, D_normal was measured at the most distal, parallel, non-tortuous normal segment of the dominant vertebral artery; if the entire middle cerebral artery was diseased, D_normal was measured at the most distal, parallel segment of the supraclinoid carotid artery; if the entire intracranial vertebral artery was diseased, D_normal was measured at the most distal, parallel, non-tortuous normal segment of the extracranial vertebral artery. Because of the variability of vasculature size, slight magnification differences, and subtle differences in anteroposterior projection, the contralateral circulation was not used as the “normal” reference measurements.

Measurement of the intracranial carotid artery required a slightly different approach because the caliber of this artery often gets slightly smaller after the origin of the ophthalmic artery, and measuring the normal cavernous portion of the intracranial carotid artery can be difficult because of the tortuosity of these segments. With this in mind, D_normal for the precavernous, cavernous, and postcavernous stenoses was measured at the widest, non-tortuous, normal portion of the petrous carotid artery that had parallel margins (first choice). If the entire petrous carotid was diseased, the most distal, parallel part of the extracranial internal carotid artery was substituted (second choice). Figure 1 illustrates how these rules were applied for measuring a stenosis of the carotid siphon and basilar artery.

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fig 1.

WASID: Warfarin-Aspirin Symptomatic Intracranial Disease. The WASID method is used in the multicenter clinical trial assessing the efficacy of warfarin versus aspirin in cases of symptomatic intracranial stenosis

If tandem intracranial lesions were present (eg, distal vertebral and mid-basilar), percent stenosis of both sites was measured and the more severe stenosis was selected. When a “gap sign” was present (ie, the lumen of the vessel could not be visualized at the site of severe stenosis), D_stenosis could not be measured with calipers. In these cases, percent stenosis was defined as 99% luminal stenosis.

Angiogram Selection, Measuring Technique

After undergoing detailed training in the measurement criteria outlined above, three neuroradiologists independently evaluated 24 angiograms showing stenoses of 24 diseased intracranial arteries: nine carotid, seven middle cerebral, five basilar, and three vertebral arteries. The angiograms were chosen by reviewing the radiology log reports at Emory University Hospital between March and July 1996. Those angiograms with at least 50% stenosis of a major intracranial artery by “eye ball” estimation were selected. None of the neuroradiologists participated in the selection process. The radiographic view (anteroposterior or lateral) that revealed the highest degree of stenosis of each arterial lesion was provided to each reader. After obtaining a single measurement of D_stenosis and D_normal, the percent diameter stenosis was calculated. Four weeks later, after rules for measuring intracranial stenosis were reinforced, each observer repeated the readings, blinded to their initial measurements.

Using a hand-held electronic caliper, all measurements were made at 90° to the arterial wall, extended point type (Mitutoyo 573–225010). This caliper has two narrow tips that do not obscure the margins of the arterial wall when measurements are obtained. A liquid crystal display provides measurements to 1/100 mm. The accuracy of the caliper is within 0.025 mm. All measurements were obtained on a horizontal lighted view box using a freestanding adjustable lighted magnifying glass (×10).

Statistical Analysis

Measurements of repeatability (measuring the same film by the same reader) and reproducibility (measuring the same film by different readers) were determined by fitting a nested analysis of variance model in which the reader factor was nested within the film factor and the repeated readings of the same film were nested within the reader factor. Both factors, film and reader, were considered as random factors (4, 5).

As a measure of repeatability, we calculated confidence intervals (95%, 90%, 85%) for the difference between the two readings of the same film by the same reader. The interpretation of this measure is that the difference between two readings of the same film by the same reader would differ by no more than this amount, half the width of the confidence interval, 95%, 90%, or 85% of the time.

As a measure of reproducibility, 95%, 90%, and 85% confidence intervals were also calculated for the difference between two readings of the same film by two different readers. Similarly, the interpretation of this measure is that the difference between the two readings of the same film by different readers would differ by no more than this amount, half the width of the confidence interval, 95%, 90%, or 85% of the time.

The analysis of variance model also provided estimates of the interclass and intraclass correlation coefficients. As a descriptive measure of repeatability, we calculated the percent of films for which the two readings of a film by the same reader were within 10% of each other (intraobserver variation). Similarly, for reproducibility, we calculated the percent of films for which the two readings of the same film by different readers were within 10% of each other (interobserver variation). This was done separately for each pairing of the three readers for both the first and second readings.

Repeatability

Among the three readers, intraobserver agreement or the percent of films in which the difference in percent stenosis between the first and second reading of a film by the same reader were less than 10% varied from 83% to 100% (81% to 100% excluding gap sign films) (Table 1). The intraclass correlation coefficient was 0.93. The estimate of the standard deviation of repeated readings of a film by the same reader based on the analysis of variance model was 3.9.

Based on confidence intervals of 95%, 90%, and 85%, estimates of repeatability were 10.8%, 9%, and 6.8%, respectively. This indicated that 95%, 90%, and 85% of the time, we would expect the difference in measurements of percent stenosis between repeated measurements of the same angiogram by the same reader to be less than 10.8%, 9%, and 6.8%, respectively.

Reproducibility

Among the pairings of the three readers, interobserver agreement or the percent of films in which the difference in percent stenosis measured by two readers was less than 10% varied from 71% to 100% (67% to 100% excluding gap sign films) (Table 2). The interclass correlation coefficient among the three readers was .85 for the first reading and .87 for the second reading.

Based on confidence intervals of 95%, 90%, and 85%, estimates of repeatability were 15.7%, 13.2%, and 11.7%, respectively. This indicated that 95%, 90%, and 85% of the time we would expect the difference in measurements of percent stenosis of the same film by different readers to be less than 15.7%, 13.2%, and 11.7%, respectively.