RT Journal Article
SR Electronic
T1 In Vitro Assessment of Vascular Injury following Stent Retriever Retraction in Clinically Relevant Endothelialized Silicone Models
JF American Journal of Neuroradiology
JO Am. J. Neuroradiol.
FD American Society of Neuroradiology
SP 517
OP 522
DO 10.3174/ajnr.A8495
VO 46
IS 3
A1 Starr, Isabelle
A1 Oen, Harrison
A1 McCulloch, Alyssa
A1 Frenklakh, Sergey
A1 Grandfield, Ryan
A1 Choe, Hana
A1 Cardinal, Kristen O’Halloran
YR 2025
UL http://www.ajnr.org/content/46/3/517.abstract
AB Mechanical thrombectomy devices have potential to injure the vessel during treatment of acute ischemic stroke. The goal of the current work was to tailor in vitro endothelialized silicone models for stent retriever assessment and to evaluate endothelial injury following treatment by various stent retriever designs and sizes. Clinically relevant neurovascular geometries were first modeled out of silicone, then sterilized, coated with fibronectin, placed in bioreactors, seeded with human endothelial cells, and cultivated under flow. Several sizes of 2 different commercially available stent retrievers were then deployed in, and retracted through, vessels. Vessels were immediately harvested and stained. Endothelial injury, identified as denudation, was quantified by using ImageJ. Results illustrated that endothelial injury ranged from 16%–18% in wire/microcatheter-only treated vessels, 37%–61% in 1-pass treatments, and 52%–70% in 2-pass treatments. Overall, this work showcases an in vitro approach for early stage assessment of the extent and location of vascular injury following stent retriever retraction.