RT Journal Article
SR Electronic
T1 Lysolecithin-induced demyelination in primates: preliminary in vivo study with MR and magnetization transfer.
JF American Journal of Neuroradiology
JO Am. J. Neuroradiol.
FD American Society of Neuroradiology
SP 225
OP 231
VO 16
IS 2
A1 V Dousset
A1 B Brochet
A1 A Vital
A1 C Gross
A1 A Benazzouz
A1 A Boullerne
A1 A M Bidabe
A1 A M Gin
A1 J M Caille
YR 1995
UL http://www.ajnr.org/content/16/2/225.abstract
AB PURPOSE To study bystander demyelination in multiple sclerosis with an experimental in vivo model of toxic demyelination. METHODS Toxic demyelinating lesions were created in two monkeys by injection of lysophosphatidylcholine in the centrum semiovale. Follow-up was done clinically and with serial MR studies, including T2-weighted and gadolinium-enhanced T1-weighted images and measurement of magnetization transfer ratio, until the animals were killed at days 14 and 34, respectively. Light and electron microscopy analysis was compared with MR data. RESULTS Interval measurement of magnetization transfer ratio during the course of the experiment revealed a maximum decrease at day 7 to day 8, associated with the greatest clinical manifestations. The lowest values of magnetization transfer ratio correlated with histopathologic findings of myelin and axon destruction. Magnetization transfer ratio measurements appear to be sensitive to macromolecular destruction and specifically to membrane disorganization. At no time was gadolinium enhancement observed in this model of toxic demyelination. CONCLUSION Preliminary results of this study indicated that magnetization transfer is a good technique to follow in vivo matrix destruction in brain parenchyma lesions. The results suggest also that phases of toxic demyelination in multiple sclerosis might not show gadolinium enhancement. Differentiation between demyelinating activity and associated inflammation in multiple sclerosis lesions should be considered in further in vivo work.