Summary
Frozen sections of cholesteatomas were compared with postauricular and auditory canal skin and studied using histochemical methods. Several dehydrogenases, lysosomal enzymes and proteolytic activity were studied. Lactate and malate dehydrogenase activity was very strong in the epithelium and subepithelial tissue of cholesteatomas; no succinate dehydrogenase activity was observed. Esterase and acid phosphatase activity was prominent in cells of granulation tissue. Considerable proteolytic activity was observed in these cells and one specimen showed possible extracellular activity. Strong evidence of fat was found in the granulation tissue, partiuclarly at the interface of granulation tissue and bone. In postauricular and auditory canal skin, enzyme activity was generally weaker; no evidence of fat was found. The findings are discussed in the light of other investigations on the importance of enzyme activity in bone destruction associated with cholesteatoma.
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References
Abramson M (1969) Collagenolytic activity in middle ear cholesteatoma. Ann Otol Rhinol Laryngol 78: 112–124
Abramson M, Gross J (1971) Further studies on a collagenase in middle ear cholesteatoma. Ann Otol Rhinol Laryngol 80: 177–185
Abramson M, Huang CC (1977) Localization of collagenase in human middle ear cholesteatoma. Laryngoscope 87: 771–791
Arnold M (1968) Histochemie-Einführung in Grundlagen und Prinzipien der Methoden. Springer, Berlin Heidelberg New York
Barka T, Anderson PJ (1965) Histochemistry — theory, practice, and bibliography. Hoeber Medical Division, Harper and Row, Publishers Incorporated
Brodmann G, Giebel W (1978) Histochemische Untersuchungen zur Funktion und Verteilung der Boettcherschen Zellen in der Goldhamstercochlea. Arch ORL (Berl) 220: 105–116
Burstone MS (1957) The cytochemical localization of esterase. J Nat Cancer Inst 18: 167–172
Cruveilhier J (1829) Anatomie pathologique du Corps Humain. Vol. 1, JB Bailliere, p 349 Paris France
Gantz BJ, Maynard J (1982) Ultrastructural evaluation of biochemical events of bone resorption in human chronic otitis media. Am J Otol 3: 279–283
Gantz BJ, Maynard J, Bumsted RM, Huang CC, Abramson M (1979) Bone resorption in chronic otitis media. Ann Otol 88: 693–700
Harris AJ (1962) Cholesteatosis and chronic otitis media. Laryngoscope 72: 954–980
Mueller J (1838) Über den feineren Bau und die Formen der krankhaften Geschwülste. G Reimer, p 50 Berlin Germany
Palva T, Forsen R, Raunio V, Palva A (1970) Lactate dehydrogenase pattern in middle ear mucosa. Pract Otorhinolaryngol 32: 129–136
Palva T, Raunio V, Forsen R, Palva A (1971) Esterases of postauricular and ear canal skin, compared with cholesteatoma epithelium. Acta Otolaryngol 72: 329–335
Palva T, Raunio V, Nousiainen R (1973) Alkaline and acid phosphatase activity in postauricular skin and cholesteatoma epithelium. Acta Otolaryngol 75: 227–231
Romeis B (1968) Mikroskopische Technik. R. Oldenburg-Verlag, 16. Aufl. München Wien
Thomsen J, Jorgensen MB, Bretlau P, Kristensen HK (1974) Bone resorption in chronic otitis media. A histological and ultrastructural study. II. Cholesteatoma. J Laryngol Otol 88: 983–992
Thomsen J, Bretlau P, Kristensen HR (1975) Bone resorption in chronic otitis media. Acta Otolaryngol 79: 400–408
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This study was supported by the „Deutsche Forschungsgemeinschaft“
This article contains a part of the doctoral thesis written by Thomas Breinlich
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Breinlich, T., Giebel, W. Histochemical studies of cholesteatoma. Arch Otorhinolaryngol 240, 95–101 (1984). https://doi.org/10.1007/BF00464352
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DOI: https://doi.org/10.1007/BF00464352