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Brain development:1H magnetic resonance spectroscopy of rat brain extracts compared with chromatographic methods

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Abstract

We compared in vitro1H magnetic resonance spectroscopy (MRS) measurements of rat brain extracts (rats: 2–56 days old) with chromatographic measurements and in a further step also with results of in vivo MRS. The following substances can be reliably measured in brain extracts by in vitro MRS: N-acetylaspartate (NAA), total creatine (Cr), phosphorylethanoloamine (PE), taurine (Tau), glutamate (Glu), glutamine (Gln), γ-aminobutyrate (GABA) and alanine (Ala). Two different methods of MRS data evaluation compared with chromatographic data on Cr and NAA are shown. During development of the rat from day 2–56 brain concentrations of PE, Tau and Ala decrease, those of NAA, Cr, Glu and Gln increase, while GABA does not change. The developmental patterns of these substances are the same, whether measured by in vitro MRS or by chromatographic methods. Quantification of NAA, Cr, Tau, GABA and PE leads to the same results with both methods, while Glu, Gln and Ala concentrations determined by in vitro MRS are apparently lower than those measured chemically. The NAA/Cr ratios of 7 to 35-day-old rats were determined by in vivo1H MRS. These results correlate with chromatographic and in vitro data. Using appropriate methods in the in vivo and in vitro MR-technique, the obtained data compare well with the chromatographic results.

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Authors and Affiliations

  1. Department of Pediatrics, University of Berne, CH-3010, Berne, Switzerland

    R. Burri & N. Herschkowitz

  2. Institute of Organic Chemistry, University of Berne, CH-3010, Berne, Switzerland

    P. Bigler & P. Straehl

  3. NMR Unit of the Medical Faculty, University of Berne, CH-3010, Berne, Switzerland

    S. Posse

  4. Department of Clinical Chemistry, Inselspital, University of Berne, CH-3010, Berne, Switzerland

    J-P Colombo

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  1. R. Burri
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  2. P. Bigler
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  3. P. Straehl
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  4. S. Posse
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  5. J-P Colombo
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  6. N. Herschkowitz
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Burri, R., Bigler, P., Straehl, P. et al. Brain development:1H magnetic resonance spectroscopy of rat brain extracts compared with chromatographic methods. Neurochem Res 15, 1009–1016 (1990). https://doi.org/10.1007/BF00965747

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