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Microstructural analysis of the effects of incorporation of myelin basic protein in phospholipid layers

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Abstract

We report an X-ray reflectivity study on the effects of adsorption of myelin basic protein (MBP) on Langmuir monolayers and on deposited Langmuir–Schaefer multilayers of the phospholipid dipalmitoyl phosphatidylglycerol (DPPG). We provide for the first time, direct microscopic evidence on the destructuring effects of MBP leading to plasticity of the DPPG layers supporting commonly accepted models of the stabilizing role of MBP in the myelin membrane. We also show how protein adsorption onto the layer is determined both by electrostatic and nonspecific hydrophobic interactions.

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Authors and Affiliations

  1. Dipartmento di Fisica e Istituto Nazionale per la Fisica della Materia, Universita‘ di Parma, Parco Area delle Scienze 7a, 43100, Parma, Italy

    L. Cristofolini, M. P. Fontana & F. Serra

  2. Dipartimento di Biochimica e Biologia Molecolare, Universita‘ di Bari, Via Orabona 4, 70126, Bari, Italy

    A. Fasano

  3. Dipartimento di Biologia, Difesa, Biotecnologie Agro-Forestali, Universita‘ della Basilicata, Campus di Macchia Romana, 85100, Potenza, Italy

    P. Riccio

  4. European Synchrotron Radiation Facility, Avenue des Martyrs, Grenoble, France

    O. Konovalov

  5. Istituto Nazionale per la Fisica della Materia, Centro Ricerca e Sviluppo SOFT, Rome, Italy

    L. Cristofolini & M. P. Fontana

Authors
  1. L. Cristofolini
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  2. M. P. Fontana
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  3. F. Serra
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  4. A. Fasano
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  5. P. Riccio
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  6. O. Konovalov
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Correspondence to L. Cristofolini.

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Cristofolini, L., Fontana, M.P., Serra, F. et al. Microstructural analysis of the effects of incorporation of myelin basic protein in phospholipid layers. Eur Biophys J 34, 1041–1048 (2005). https://doi.org/10.1007/s00249-005-0489-5

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  • DOI: https://doi.org/10.1007/s00249-005-0489-5

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