Original contribution
Localized 2D J-resolved 1H MR spectroscopy: Strong coupling effects in vitro and in vivo

https://doi.org/10.1016/0730-725X(95)00031-BGet rights and content

Abstract

A two-dimensional (2D) J-resolved MR spectroscopy sequence (2D J-PRESS), fully localized in three dimensions, has been implemented on a whole-body MR scanner. A modified PRESS sequence with [90°–180°-t1/2–180°-t1/2-acquisition] was used for voxel localization. An incremental delay (t12) was added to the intervals before and after the last slice-selective 180° RF pulse to monitor the J-evolution in a localized 2D MR spectrum. Spectra were recorded with phantoms containing common cerebral metabolites-alanine, N-acetyl aspartate, glutamine, glutamate, taurine, myo-inositol, glucose, aspartate, GABA, and choline at 50 mM. In conformity with previously reported results, additional cross-peaks due to strong coupling were monitored in many metabolites. A brain phantom was developed to mimic the gray matter of human brain with the metabolites at physiological concentrations (0.5–12 mM). In vivo 2D J-PRESS spectra (n = 18) of healthy human brain were in conformity with those recorded from the brain phantom.

References (33)

  • E.J. Delikatny et al.

    The Effect of altering time domains and window functions in two-dimensional proton COSY spectra of biological specimens

    J. Magn. Reson.

    (1991)
  • G. Otting et al.

    Origin of t1 and t2 ridges in 2D NMR spectra and procedures for suppression

    J. Magn. Reson.

    (1986)
  • D. Marion et al.

    Baseline distortions in real FT NMR spectra

    J. Magn. Reson.

    (1988)
  • M. Deriche et al.

    Elimination of water signal by post processing

    J. Magn. Reson.

    (1993)
  • R. Kreis et al.

    Absolute quantitation of water and metabolites in the human brain: II Metabolite concentrations

    J. Magn. Reson.

    (1993)
  • E.L. Hahn et al.

    Spin echo measurements of nuclear spin coupling in molecules

    Phys. Rev.

    (1952)
  • Cited by (112)

    • Effects of noise and linewidth on in vivo analysis of glutamate at 3 T

      2020, Journal of Magnetic Resonance
      Citation Excerpt :

      The latter approach is not applicable to single TE techniques, which are unable to derive T2 values. J-resolved spectroscopy, also known as JPRESS [6], acquires a series of different TE spectral data which can be transformed into a 2D spectrum; one dimension gives the spectrum of chemical shifts, and the second dimension shows J-coupling information. Metabolite resonance lines can then be identified concurrently by their chemical shifts and J-coupling information, with significantly improved spectral resolution.

    View all citing articles on Scopus
    View full text