Elsevier

Developmental Brain Research

Volume 132, Issue 2, 31 December 2001, Pages 175-178
Developmental Brain Research

Short communication
AMPA-Induced suppression of oligodendroglial gene expression in neonatal rat brain

https://doi.org/10.1016/S0165-3806(01)00306-6Get rights and content

Abstract

Oligodendroglia are susceptible to α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated excitotoxicity. We hypothesized that analysis of oligodendroglial gene expression would provide a sensitive indicator of acute excitotoxic oligodendroglial injury in the immature brain. AMPA (2 nmol) was injected intracerebroventricularly in 7-day-old rats. Proteolipid protein and myelin basic protein encoding mRNAs were evaluated 24–48 h later by semi-quantitative reverse transcription–polymerase chain reaction assays. AMPA markedly suppressed brain tissue content of oligodendroglial mRNAs.

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Acknowledgements

Supported by USPHS grants NS 35059 and 40835 (to F.S.S. and J.D.E.B.). Drs. R. Skoff and L. Carlock provided helpful advice regarding the design of PLP and MBP primers.

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