Peculiarities of axonal transport of steroid hormones (hydrocortisone, testosterone) in spinal root fibres of adult and old rats
Section snippets
Experimental procedures
The experiments were performed on male Wistar rats aged six to 11 and 25–28 months kept at 22–26°C. The animals were anaesthetized with sodium pentobarbital (30 mg/kg, i.p.). Axonal transport was studied in the L5–L6 ventral (anterograde flow) and dorsal (retrograde flow) spinal roots using the technique described elsewhere.8 Benzene-ethanol solution of [3H]hydrocortisone and benzene solution of [14C]testosterone were injected into the gray matter of L5–L6 segments in a dose of 7–8 μl. It
Results
Studying the distribution of labelled hydrocortisone in the spinal roots of adult rats showed that this hormone becomes involved rather quickly in both the anterograde (motoneuronal axons) and retrograde (central processes of the spinal ganglia neurons) AT.
Ten minutes after the hormone administration into the spinal cord, an elevated frequency of disintegrations, significantly exceeding their background level, was noted in the root fragments located 21–24 mm away from the spinal cord, whereas 15
Discussion
The results of our experiments indicated that steroid hormones ([3H]hydrocortisone and [14C]testosterone) become involved in AT and are transported with very high velocity along the fibres of the ventral and dorsal spinal roots in adult rats. The calculated maximum velocities of anterograde and retrograde transports of [3H]hydrocortisone were practically equal to each other and amounted to about 3000 mm/day. The velocities of anterograde and retrograde transports of [14C]testosterone were 1.5
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