The AMP-activated protein kinase (AMPK) exists as a heterotrimetric complex comprising a
catalytic α subunit and non-catalytic β and γ subunits. Under conditions of hypoxia, exercise, …

… RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those
produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed …

… ) contain a unique conserved region in their N-terminal half (the RNC domain), followed by
three HEAT repeats and seven WD repeats near the C terminus. The binding of TOR to raptor …

Traditional adaptive methods that compensate for PVT variations need safety margins and
cannot respond to rapid environmental changes. In this paper, we present a design (RazorII) …

… ) or with 1 mm AICAR for the indicated time periods (panels C and D). In panels A and C,
activities of p70 S6k were assayed after immunoprecipitation as described under Experimental …

… at 38C followed by a 30-min chase reaction at 38C. Spheroplasts were lysed in osmotic lysis
buffer C at … at 5,000 g for 5 min at 4C. The P5 pellet was resuspended in osmotic lysis buffer …

… custom testing software written in Perl and C programming languages. The testing software
and JTAG scan controller load the binary for the C-compiled benchmarks and the program …

… indicated concentrations of rapamycin for 90 min at 30 C in buffer C (10 mm HEPES [pH 7.5],
… Then the beads were washed four times with buffer C and twice with buffer B. The washed …

… 4 C. The anti-mTOR immunocomplex and the anti-recombinant p70α immunocomplex were
each prepared using separate supernatants incubated 2 h at 4 C … min at 4 C. Normal mouse …

… mTOR contains a kinase domain near its C-termini, most closely related to the
phosphatidylinositol kinase (PIK)-related kinase family, which includes ATM, ATR, and DNA-PK …