The type III secretion injectisome is a complex nanomachine that allows bacteria to deliver
protein effectors across eukaryotic cellular membranes. In recent years, significant progress …

… Both nonopsonized bacteria and bacteria opsonized with rabbit anti-Yersinia immunoglobulin
G were able to inhibit their uptake by J774A.1 macrophages, indicating thatYersinia can …

… Work in Guy Cornelis's laboratory is supported by the Belgian Fonds National de la Recherche
Scientifique Médicale (Convention 3.4595.97), the Direction Générale de la Recherche …

… Vanooteghem for critical reading of the manuscript, and G. Rufflard for excellent technical
assistance. … and Wauters, G.: Correlation of serological and biochemical groupings of Yersinia …

The Yop virulon enables Yersinia spp. (Y. pestis, Y. pseudotuberculosis and Y. enterocolitica)
to survive and multiply in the lymphoid tissues of their host. It is an integrated system …

… It also involves individual cytoplasmic chaperones that presumably act as pilots to target
nascent proteins to the secretion apparatus (Wattiau and Cornelis. 1993; Wattiau et a/., 1994). …

… When Yersinia are opsonized with the complement fragment C3b or with immunoglobulin
G, the removal of YopE seems to have little effect on phagocytosis by J774 macrophages, …

Pathogenic Yersinia spp (Yersinia pestis, Yersinia pseudotuberculosis, and Yersinia
enterocolitica) have evolved an exquisite method for delivering powerful effectors into cells of the …

… mM HCl/0.1% Triton X-100) as described by Sory and Cornelis (20), and cAMP was assayed
by an enzyme immunoassay (Biotrak… We thank G. Wauters for the gift of strain E40 and J.-C. …

… Cells were collected by centrifugation at either 8,000 x g for 10 min or at 1,900 x g for 30 min…
g of(NH4)2SO4. Proteins were subsequently pelleted by a 30-min centrifugation at 1,900 x g…