… et al., 1997; Ishizaki et al., 1997], (2) the C-terminal segment interacts with and inhibits the
constitutively active catalytic fragment both in vitro and in vivo [Amano et al., 1999], and (3) an …

▪ Abstract Members of the Rho family of small Ras-like GTPases—including RhoA, -B, and -C,
Rac1 and -2, and Cdc42—exhibit guanine nucleotide-binding activity and function as …

Hypercontraction or abnormal contraction of vascular smooth muscle is a major cause of
diseases such as hypertension and vasospasm of the coronary and cerebral arteries. A better …

The small GTPase Rho is implicated in physiological functions associated with actin-myosin
filaments such as cytokinesis, cell motility, and smooth muscle contraction. We have recently …

… We thank M. Fujioka and J. Tanaka for preparing polyclonal antibodies to MBS and the 20-…
Hamajima for preparing NIH 3T3 cell lines, M. Inagaki for valuable discussions, and M. …

… , 0.05% TritonX-100) containing 6 M guanidium hydrochloride, followed by incubation for
3 min in buffer B containing 3 M guanidium hydrochloride. This was repeated four times. The …

The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress
fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as …

The ezrin/radixin/moesin (ERM) proteins are involved in actin filament/plasma membrane
interaction that is regulated by Rho. We examined whether ERM proteins are directly …

… containing 50 mm Tris-HCl, pH 7.2, 400 mm NaCl, 2 mm EGTA, 1 mm EDTA, 1 mm DTT, 0.1
μm p-amidinophenylmethanesulfonyl fluoride hydrochloride, 10 μg/ml leupeptin, and 1 mm …

… However, the endogenous kinase appears to be distinct from Rho-kinase because some
characters of the endogenous kinase are different from those of Rho-kinase (Amano, M., and K. …