Article Figures & Data
Figures
- Fig 1.
Representative pathologic findings in the same animal (S05) at the same location observed in vivo (T2-weighted, TR/effective − TE = 2000 ms/62.5 ms; NA = 6), in vitro (TR/TE = 2500 ms/20 ms; NA = 8), and in histologic sections. Many of the same pathologic details are visible with all three methods albeit with different levels of resolution. In vivo and in vitro images are displayed with image size = 0.92 cm × 0.77 cm (in vivo) and 0.50 cm × 0.50 cm (in vitro). Letters label the most distinctive anatomic and pathologic characteristics observed in the three images as follows: A indicates expanded central canal; B, injection track; C, dorsal root ganglion; D, dorsal root; E, hemorrhage at injury site; F, white matter; G, gray matter; H, ventral root; I, cavity; J, anterior spinal vessel. In vivo images show cavitation as hyperintense signals due to T2 weighting, whereas, on in vitro images, cavities appear isointense in these proton attenuation-weighted images.
- Fig 2.
A, In vivo (T2-weighted, TR/effective TE = 2000 ms/62.5 ms; NA = 6); and, B, in vitro (TR/TE = 2500 ms/20 ms; NA = 8) sagittal images for all QUIS-injured animals. Images are oriented with rostral at the top and dorsal on the right. Arrows represent the location of the epicenter of the injury. The epicenter was defined as the region of maximal pathologic damage. Displayed image size = 0.77 cm × 2.15 cm (in vivo) and 0.40 cm × 1.50 cm (in vitro). Animals designated S01, S02, S04, S05, and S06 received QL2, whereas animals designated X01 and X03 received QL1. The vertebral column and laminectomy site were easily observed in both in vivo and in vitro sagittal images.
- Fig 3.
A, In vivo (T2-weighted, TR/effective − TE = 2000 ms/62.5 ms; NA = 6); and, B, in vitro (TR/TE = 2500 ms/20 ms; NA = 8) transverse images for all QUIS-injured animals. Images are sampled from the epicenter of the injury (arrows in Fig 2) and show comparable pathologic changes between methods. See Fig 1 for labeling of anatomic and pathologic findings. Images are oriented with dorsal at the top. L, left side of cord; R, right side of cord, for all images. Displayed image size = 0.92 cm × 0.77 cm (in vivo) and 0.50 cm × 0.50 cm (in vitro). A, Hypointense signals correlated with hemorrhage (see “Histologic Findings”) and hyperintense signals correlated with the presence of fluid filled cavities. For example, notice the hyperintense expanded central canal in the cord of animals S01, S02, and S04–S06 and the hyperintense cavity in the gray matter on the right side of the cord in animal S02. B, Hypointense regions were observed bilaterally in the gray matter in all QL2 animals (S01, S02, and S04–S06), whereas, in QL1 animals, evidence of hemorrhage was observed only ipsilateral to the side of QUIS injection (X01 and X03). In addition, hypointense areas were observed in the dorsal columns in all the QUIS-injured animals except S01. Central canal expansion was clearly defined in four of the five QL2 animals (S01 and S04–S06), and injection tracks were visible in all injured animals. Cavity formation was also seen in all QL2 animals bilaterally.
- Fig 4.
Total injury lengths determined by using in vivo, in vitro, and histologic methods for each QUIS-injured animal. Total injury lengths in millimeters reflect the presence of excitotoxic tissue damage. No significant differences are observed between in vitro and histology (P > .05). Significant differences are observed between in vivo and in vitro/histology (P < .05), but these differences are within the range of section thickness difference between in vivo and in vitro images. A single asterisk denotes the QL2 animal, and double asterisks denote the QL1 animal.
- Fig 5.
Summary of in vivo, in vitro, and histologic data collected for a representative QL2 animal (S06). Representative sections (1–5) are sampled from the same rostrocaudal location in the spinal cord with each method used. Image sizes are the same as in Figs 2 and 3. See Fig 1, for labeling of anatomic and pathologic findings. Although sagittal images show the rostral-caudal extent of the injury, transverse sections provide greater detail of the pathologic changes associated with this injury model.
- Fig 6.
Summary of in vivo, in vitro, and histologic data collected for a representative QL1 animal (X03). Representative sections (1–5) are sampled with each method from the same rostrocaudal location in the spinal cord. Image sizes are the same as in Figs 2 and 3. See Fig 1, for labeling of anatomic and pathologic findings. Although sagittal images show the rostral-caudal extent of the injury, transverse sections provide greater detail of the pathologic changes associated with this injury model.
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